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PARP1 蛋白
PARP1
宿主: 人
宿主: Baculovirus
Recombinant
> 95 %
WB, ELISA
产品细节
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PARP1
(Poly (ADP-Ribose) Polymerase 1 (PARP1))
蛋白类型
Recombinant
宿主
All origins for PARP1 蛋白
人
资源
All sources for PARP1 蛋白
Baculovirus
应用范围
Western Blotting (WB), ELISA
产品特性
PARP1 Automodified is highly purified human PARP1, expressed in a baculovirus expression system, to which NAD+ and cofactors have been added to allow PARP automodification by poly(ADP-ribose). The PARP1 is lyophilized from its native state, and upon reconstitution with water, it retains residual poly ADP-ribosylation enzymatic activity. It is useful as a standard for ELISA, SDS-PAGE, Western blotting, and in other assays. Full-length hPARP1
纯化方法
Affinity chromatography
纯度
> 95 %
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使用细节
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应用备注
100 ng/lane WB, 10 ng/well ELISA
限制
仅限研究用
贮存及处理
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状态
Lyophilized
溶解方式
Reconsitute in dH2O
储存条件
-20 °C
引用
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Zhang, Wang, Ding, Yu : "Site-specific characterization of the Asp- and Glu-ADP-ribosylated proteome. " in: Nature methods , Vol. 10 , Issue 10 , pp. 981-4 , (2013 ) (PubMed ).
目标详细情况
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抗原
PARP1
(Poly (ADP-Ribose) Polymerase 1 (PARP1))
别名
Poly ADP ribose Polymerase 1 (PARP1 产品 )
别名
ADPRT Protein, ADPRT 1 Protein, ADPRT1 Protein, ARTD1 Protein, PARP Protein, PARP-1 Protein, PPOL Protein, pADPRT-1 Protein, Adprt Protein, Parp-1 Protein, 5830444G22Rik Protein, AI893648 Protein, Adprp Protein, Adprt1 Protein, C80510 Protein, parp-1 Protein, sPARP-1 Protein, BEST:LD21673 Protein, CG17685 Protein, CG17696 Protein, CG17718 Protein, CG40411 Protein, D.PARP Protein, Dm.pARTa Protein, Dmel\\CG40411 Protein, LD21673.3prime Protein, PARP1 Protein, dPARP Protein, parp Protein, adprt1 Protein, padprt-1 Protein, ppol Protein, si:dkey-206f10.3 Protein, wu:fc60f12 Protein, zgc:110092 Protein, poly(ADP-ribose) polymerase 1 Protein, poly (ADP-ribose) polymerase 1 Protein, poly (ADP-ribose) polymerase family, member 1 Protein, Poly-(ADP-ribose) polymerase Protein, poly(ADP-ribose) polymerase 1 L homeolog Protein, PARP1 Protein, Parp1 Protein, Parp Protein, parp1.L Protein, parp1 Protein
背景
Poly (ADP-ribose) polymerase-1 (PARP1) is an abundant and ubiquitous nuclear enzyme that catalyzes the NAD(+)-dependent addition of ADP-ribose polymers on a variety of nuclear proteins.
分子量
>113 kDa
途径
Apoptosis , Caspase Cascade in Apoptosis , DNA Damage Repair , Production of Molecular Mediator of Immune Response , Maintenance of Protein Location
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