LacI (AA 1-360) protein (His tag)
宿主: 人
宿主: 大肠杆菌（E. Coli）
Recombinant
> 95 % as determined by reducing SDS-PAGE.
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- 抗原
- LacI
- 蛋白类型
- Recombinant
- 产品特性
- AA 1-360
- 宿主
- 人
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资源
- 大肠杆菌（E. Coli）
- 标记
- His tag
- 序列
- MKPVTLYDVA EYAGVSYQTV SRVVNQASHV SAKTREKVEA AMAELNYIPN RVAQQLAGKQ SLLIGVATSS LALHAPSQIV AAIKSRADQL GASVVVSMVE RSGVEACKAA VHNLLAQRVS GLIINYPLDD QDAIAVEAAC TNVPALFLDV SDQTPINSII FSHEDGTRLG VEHLVALGHQ QIALLAGPLS SVSARLRLAG WHKYLTRNQI QPIAEREGDW SAMSGFQQTM QMLNEGIVPT AMLVANDQMA LGAMRAITES GLRVGADISV VGYDDTEDSS CYIPPLTTIK QDFRLLGQTS VDRLLQLSQG QAVKGNQLLP VSLVKRKTTL APNTQTASPR ALADSLMQLA RQVSRLESGQ HHHHHH
- 产品特性
- Recombinant E.coli Lactose operon repressor/LacI is produced with our E. coli expression system. The target protein is expressed with sequence (Met1-Gln360) of E.coli LacI fused with a 6His tag at the C-terminus.
- 纯度
- > 95 % as determined by reducing SDS-PAGE.
- 过滤
- 0.2 μm filtered
- 内毒素水平
- Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
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- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- Supplied as a 0.2 μm filtered solution of 20 mM Tris, 300 mM NaCl, 5 mM DTT pH 8.0 .
- 注意事项
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Always centrifuge tubes before opening.
Do not mix by vortex or pipetting.
Please aliquot to minimize freeze-thaw cycles. - 储存条件
- -80 °C
- 储存方法
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Store at < -20°C, stable for 6 months after receipt.
Please minimize freeze-thaw cycles. - 有效期
- 6 months
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- 抗原
- LacI
- 别名
- ECK0342 Protein, JW0336 Protein, lactose-inducible lac operon transcriptional repressor Protein, lacI Protein
- Sub Type
- Fusionprotein
- 背景
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Lactose operon repressor (LacI) contains one HTH lacI-type DNA-binding domain, functions as a homotetramer. Lactose operon repressor as a repressor of the lactose operon, which also as an inducer, binds allolactose. If remove residues 1-59, resulting the loss of DNA-binding activity but retains tetrameric structure and inducer-binding activity. If delete residues 340-360, resulting the loss of tetramer formation, but retains dimer formation, inducer-binding activity, and DNA-binding activity.
Alternative Names: Lactose operon repressor, LacI - 分子量
- 39.4 kDa
- UniProt
- P03023
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