Recombinant Human Ubiquitin-Conjugating Enzyme E2 Variant 2/UBE2V2 is produced with our E. coli expression system. The target protein is expressed with sequence (Met1-Asn145) of Human UBE2V2 fused with a His tag at the N-terminus.
纯度
> 95 % as determined by reducing SDS-PAGE.
过滤
0.2 μm filtered
内毒素水平
Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
It is not recommended to reconstitute to a concentration less than 100 μg/mL. Dissolve the lyophilized protein in ddH2O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
缓冲液
Supplied as a 0.2 μm filtered solution of 50mm HEPES,150 mM NaCl, pH 7.0.
注意事项
Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
储存条件
-80 °C
储存方法
Store at < -20°C, stable for 6 months after receipt. Please minimize freeze-thaw cycles.
Ubiquitin-Conjugating Enzyme E2 Variant 2 (UBE2V2) is an enzyme that belongs to the ubiquitin-conjugating enzyme family. UBE2V2 can be detected in the placenta, colon, liver, and skin. It forms a heterodimer with UBE2N. The UBE2V2/UBE2N heterodimer catalyzes the synthesis of non-canonical poly-ubiquitin chains and which leads to protein degradation by the proteasome. UBE2V2 mediates transcriptional activation of target genes. It plays a role in the control of progress through the cell cycle and differentiation. It also plays a role in the error-free DNA repair pathway and contributes to the survival of cells after DNA damage. Alternative Names: Ubiquitin-Conjugating Enzyme E2 Variant 2, DDVit 1, Enterocyte Differentiation-Associated Factor 1, EDAF-1, Enterocyte Differentiation-Promoting Factor 1, EDPF-1, MMS2 Homolog, Vitamin D3-Inducible Protein, UBE2V2, MMS2, UEV2