Recombinant Human Regenerating islet-derived protein 4/REG4 is produced by our mammalian expression system in human cells. The target protein is expressed with sequence (Asp23Pro158) of Human REG4 fused with a polyhistidine tag at the C-terminus.
纯度
> 95 % as determined by reducing SDS-PAGE.
过滤
0.2 μm filtered
内毒素水平
Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
REG4
宿主: 人
宿主: HEK-293 Cells
Recombinant
> 90 % as determined by reducing SDS-PAGE.
限制
仅限研究用
状态
Lyophilized
溶解方式
It is not recommended to reconstitute to a concentration less than 100 μg/mL. Dissolve the lyophilized protein in ddH2O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
缓冲液
Lyophilized from a 0.2 μm filtered solution of 20 mM PB,150 mM NaCl, pH 7.2.
注意事项
Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
储存条件
4 °C/-20 °C/-80 °C
储存方法
Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
有效期
5 months
抗原
REG4
(Regenerating Islet-Derived Family, Member 4 (REG4))
GISP Protein, REG-IV Protein, RELP Protein, 2010002L15Rik Protein, regenerating family member 4 Protein, regenerating islet-derived family, member 4 Protein, Reg4 Protein, REG4 Protein
Sub Type
Fusionprotein
背景
REG4 is a secreted contains one C-type lectin domain, high expressed in the gastrointestinal tract, including jejunum, ileum, appendix, pancreas and small intestine. REG4 can be up-regulated by mucosal injury from active Crohn’s disease or ulcerative colitis. In the acid environment, REG4 can maintain carbohydrate recognition activity. REG4 may be involved in inflammatory and metaplastic response of the gastrointestinal epithelium. Alternative Names: Regenerating islet-derived protein 4, Gastrointestinal secretory protein, REG-like protein, Regenerating islet-derived protein IV, GISP, RELP, REG4