(1 reference)-
- 抗原 See all AHSA1 蛋白
- AHSA1 (Activator of HSP90 ATPase Activity 1 (AHSA1))
- 蛋白类型
- Recombinant
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宿主
- 人
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资源
- 大肠杆菌（E. Coli）
- 应用范围
- Western Blotting (WB), SDS-PAGE (SDS), Functional Studies (Func)
- 序列
- SHMAKWGEGD PRWIVEERAD ATNVNNWHWT ERDASNWSTD KLKTLFLAVQ VQNEEGKCEV TEVSKLDGEA SINNRKGKLI FFYEWSVKLN WTGTSKSGVQ YKGHVEIPNL SDENSVDEVE ISVSLAKDEP DTNLVALMKE EGVKLLREAM GIYISTLKTE FTQGMILPTM NGESVDPVGQ PALKTEERKA KPAPSKTQAR PVGVKIPTCK ITLKETFLTS PEELYRVFTT QELVQAFTHA PATLEADRGG KFHMVDGNVS GEFTDLVPEK HIVMKWRFKS WPEGHFATIT LTFIDKNGET ELCMEGRGIP APEEERTRQG WQRYYFEGIK QTFGYGARLF
- 特异性
- ~38 kDa
- 产品特性
- 2 µM ABIN1686714 generated a 9-fold ATPase activation of 2 µM Hsp90 (His-tagged Hsp90 beta) in 33 mM Hepes pH 7.2, 30 mM NaCl, 5 mM MgCl2, 1 mM DTT, 1.5 mM ATP in a 100 µL reaction at 37 degrees C. (This is an enzyme-linked ATP regeneration assay tracking loss of NADH absorbance at 340nm.)
- 纯化方法
- Multi-Step Purified
- 纯度
- >90%
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Activator of HSP90 ATPase Activity 1 (AHSA1) (AA 272-627) protein
AHSA1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % by SDS - PAGE SDS
Activator of HSP90 ATPase Activity 1 (AHSA1) (AA 1-338) protein (His tag)AHSA1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Activator of HSP90 ATPase Activity 1 (AHSA1) (AA 1-338) protein (His tag)AHSA1 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Activator of HSP90 ATPase Activity 1 (AHSA1) (AA 1-338) protein (GST tag)AHSA1 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Activator of HSP90 ATPase Activity 1 (AHSA1) protein (His tag)AHSA1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant >90% WB, SDS, Func
Activator of HSP90 ATPase Activity 1 (AHSA1) (AA 1-338), (full length) protein (GST tag)AHSA1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant 95 % ELISA
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 说明
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This product has been certified >90% pure using SDS PAGE analysis. 2uM ABIN1686714 generated a 9-fold ATPase activation of 2uM HSP90 (His-tagged HSP90 beta) in 33mM Hepes pH7.2, 30mM NaCl, 5mM MgCl2, 1mM DTT, 1.5mM ATP in a 100ul reaction at 37 degrees C. (This is an enzyme-linked ATP regeneration assay tracking loss of NADH absorbance at 340nm.)
- 限制
- 仅限研究用
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- 浓度
- Lot specific
- 缓冲液
- 20 mM HEPES buffer pH 7.2, 80 mM NaCl, 10 % glycerol
- 储存条件
- -20 °C
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-
: "Chemical proteomics-driven discovery of oleocanthal as an Hsp90 inhibitor." in: Chemical communications (Cambridge, England), Vol. 49, Issue 52, pp. 5844-6, (2013) (PubMed).
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: "Chemical proteomics-driven discovery of oleocanthal as an Hsp90 inhibitor." in: Chemical communications (Cambridge, England), Vol. 49, Issue 52, pp. 5844-6, (2013) (PubMed).
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- 抗原
- AHSA1 (Activator of HSP90 ATPase Activity 1 (AHSA1))
- 别名
- Aha1 (AHSA1 产品)
- 背景
- Aha1 is a member of the HSP90 cochaperone family, and is thought to stimulate HSP90 ATPase activity by competing with p23 and other co-chaperones for HSP90 binding (1, 2). It may affect a step in the endoplasmic reticulum to Golgi trafficking. Aha1 also interacts with HSPCA/HSP90 and with the cytoplasmic tail of the vesicular stomatistis virus glycoproteins (VSV G) (3). Aha1 is expressed in numerous tissues, including the brain, heart, skeletal muscle, and kidney, and at low levels, the liver and placenta. Aha1 might be a potential therapeutic strategy to increase sensitivity to HSP inhibitors (4).
- 分子量
- approx. 38 kDa
- 基因ID
- 10598
- NCBI登录号
- NP_036243
- UniProt
- O95433
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