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CD34 Protein (CD34) (C-Term, Extracellular Domain)

CD34 宿主: 小鼠 宿主: HEK-293T Cells Recombinant > 95 %, as determined by SDS-PAGE and HPLC Active
产品编号 ABIN1684672
发货至: 中国
  • 抗原 See all CD34 蛋白
    CD34
    蛋白类型
    Recombinant
    生物活性
    Active
    产品特性
    C-Term, Extracellular Domain
    宿主
    • 15
    • 5
    • 1
    • 1
    小鼠
    资源
    • 9
    • 6
    • 2
    • 2
    • 1
    • 1
    HEK-293T Cells
    特异性
    Optimized DNA sequence encoding extracellular domain of mouse CD34 including a C-terminal 6His tag was expressed in HEK293 cells.
    产品特性
    Recombinant mouse CD34 is a monomer protein consisting of70 amino acid residue subunits, due to glycosylation migrates as an approximately 50 kDa protein on SDS-PAGE.
    纯度
    > 95 %, as determined by SDS-PAGE and HPLC
    过滤
    0.2 μm filtered
    内毒素水平
    Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
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    Discover our top product CD34 蛋白
  • 说明

    Biologically active: Measured by the ability to support the adhesion of the HUVEC cell line. Coated plates with immobilized recombinant mouse CD34 ( 0.8 µg/mL) are able to adhere 40 % of cells ( 4 x 104 cells/well) after one hour at 37 °C

    限制
    仅限研究用
  • 溶解方式
    A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers
    缓冲液
    PBS solution, pH7.2.
    注意事项
    Avoid repeated freeze/thaw cycles.
    储存条件
    -20 °C
    储存方法
    The lyophilized protein is stable for at least years from date of receipt at -20 °C. Upon reconstitution, this cytokine can be stored in working aliquots at - 8 °C for one month, or at -20 °C for six months, with a carrier protein without detectable loss of activity.
    有效期
    12-24 months
  • 抗原
    CD34
    别名
    CD34 (CD34 产品)
    别名
    AU040960 Protein, CD34 molecule Protein, CD34 antigen Protein, CD34 Protein, Cd34 Protein
    背景
    Parathyroid hormone is a circulating hormone that acts as the central regulator of calcium metabolism by directly targeting bone, kidney, and intestine. The classical concept of PTH action is that it regulates serum calcium levels by stimulating bone resorption, however, intermittent administration of PTH selectively stimulates bone formation. It is now known that PTH binds to its receptor PTH1R, and activates the G protein alpha subunits. Besides PKA and PKC activation, PTH also regulates MAPKs including p42/p44 ERKs, p38, and c-Jun N-terminal kinase subtypes.
    UniProt
    Q64314
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