CD34 Protein (CD34) (C-Term, Extracellular Domain)
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- 抗原 See all CD34 蛋白
- CD34
- 蛋白类型
- Recombinant
- 生物活性
- Active
- 产品特性
- C-Term, Extracellular Domain
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宿主
- 小鼠
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资源
- HEK-293T Cells
- 特异性
- Optimized DNA sequence encoding extracellular domain of mouse CD34 including a C-terminal 6His tag was expressed in HEK293 cells.
- 产品特性
- Recombinant mouse CD34 is a monomer protein consisting of70 amino acid residue subunits, due to glycosylation migrates as an approximately 50 kDa protein on SDS-PAGE.
- 纯度
- > 95 %, as determined by SDS-PAGE and HPLC
- 过滤
- 0.2 μm filtered
- 内毒素水平
- Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
- Top Product
- Discover our top product CD34 蛋白
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- 说明
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Biologically active: Measured by the ability to support the adhesion of the HUVEC cell line. Coated plates with immobilized recombinant mouse CD34 ( 0.8 µg/mL) are able to adhere 40 % of cells ( 4 x 104 cells/well) after one hour at 37 °C
- 限制
- 仅限研究用
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- 溶解方式
- A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers
- 缓冲液
- PBS solution, pH7.2.
- 注意事项
- Avoid repeated freeze/thaw cycles.
- 储存条件
- -20 °C
- 储存方法
- The lyophilized protein is stable for at least years from date of receipt at -20 °C. Upon reconstitution, this cytokine can be stored in working aliquots at - 8 °C for one month, or at -20 °C for six months, with a carrier protein without detectable loss of activity.
- 有效期
- 12-24 months
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- 抗原
- CD34
- 别名
- CD34 (CD34 产品)
- 别名
- AU040960 Protein, CD34 molecule Protein, CD34 antigen Protein, CD34 Protein, Cd34 Protein
- 背景
- Parathyroid hormone is a circulating hormone that acts as the central regulator of calcium metabolism by directly targeting bone, kidney, and intestine. The classical concept of PTH action is that it regulates serum calcium levels by stimulating bone resorption, however, intermittent administration of PTH selectively stimulates bone formation. It is now known that PTH binds to its receptor PTH1R, and activates the G protein alpha subunits. Besides PKA and PKC activation, PTH also regulates MAPKs including p42/p44 ERKs, p38, and c-Jun N-terminal kinase subtypes.
- UniProt
- Q64314
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