Optimized DNA sequence encoding Human extracellular domain of human CD31including a C-terminal Fc tag was expressed in HEK293 cells.
纯度
> 95 %, as determined by SDS-PAGE and HPLC
过滤
0.2 μm filtered
内毒素水平
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Biological Activity Comment
Recombinant Human CD31 activity was determined by its ability to support the adhesion of Jurkat human leukemic T cells. When cells are added to recombinant human CD31 coated plates ( 3.3 μg/mL , 100 μL/well ) in the presence of PMA , approximately 50% - 65% will adhere specifically after 30 minutes at 37°C
PECAM1
宿主: 人
宿主: 人细胞
Recombinant
> 95 % as determined by reducing SDS-PAGE.
限制
仅限研究用
缓冲液
Recombinant CD31 is supplied as a 0.2 μm filtered PBS solution, pH7.2 .
注意事项
Avoid repeated freeze/thaw cycles.
储存条件
-20 °C
储存方法
The lyophilized antibody is stable for at least 1 year from date of receipt at -20 °C. Upon reconstitution, this antibody can be stored in working aliquots at - 8 °C for one month, or at -20 °C for six months without detectable loss of activity.
有效期
12 months
Kitazume, Imamaki, Kurimoto, Ogawa, Kato, Yamaguchi, Tanaka, Ishida, Ando, Kiso, Hashii, Kawasaki, Taniguchi: "Interaction of platelet endothelial cell adhesion molecule (PECAM) with α2,6-sialylated glycan regulates its cell surface residency and anti-apoptotic role." in: The Journal of biological chemistry, Vol. 289, Issue 40, pp. 27604-13, (2014) (PubMed).