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VEGF 165 (Homodimer) (Active) 蛋白

Name: VEGF 165 (Homodimer) (Active) 蛋白
SKU: ABIN1589678
Availability: OutOfStock

宿主: 人宿主: 大肠杆菌（E. Coli） Recombinant > 95 % by SDS-PAGE. Visualized by silver stain Active

(1 reference)

产品编号 ABIN1589678

发货至: 中国

Datasheet as PDF

抗原

VEGF 165

蛋白类型

Recombinant

生物活性

Active

产品特性

Homodimer
宿主
20

3

2
人
资源
12

7

2

1

1

1

1
大肠杆菌（E. Coli）

序列

APMAEGGGQN HHEVVKFMDV YQRSYCHPIE TLVDIFQEYP DEIEYIFKPS CVPLMRCGGC CNDEGLECVP TEESNITMQI MRIKPHQGQH IGEMSFLQHN KCECRPKKDR ARQENPCGPC SERRKHLFVQ DPQTCKCSCK NTDSRCKARQ LELNERTCRC DKPRR

产品特性

Length (AA): 164
Chromosomal location: 6p12
The ED50 for stimulation of cell proliferation in human umbilical vein endothelial cells by VEGF165 has been determined to be in the range of 1-5 ng/mL.

纯度

> 95 % by SDS-PAGE. Visualized by silver stain

内毒素水平

< 0.1 ng per µg of human VEGF165
VEGF 165 protein
宿主: 人宿主: HEK-293 Cells Recombinant > 95% as determined by Tris-Bis PAGE,> 95% as determined by HPLC

VEGF 165 (AA 27-191) protein (His-Avi Tag,Biotin)
宿主: 人宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE

VEGF 165 (AA 27-191) (Active) protein (His tag,AVI tag,Biotin)
宿主: 人宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Func Active

VEGF 165 (AA 27-191) protein (His-Avi Tag)
宿主: 人宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE

VEGF 165 (Homodimer) (Active) protein
宿主: 人宿主: Insect Cells Recombinant > 95 % by SDS-PAGE Active

VEGF 165 (AA 207-371) protein (His tag)
宿主: 人宿主: 大肠杆菌（E. Coli） Recombinant > 90 % by SDS - PAGE SDS

VEGF 165 protein
宿主: 人宿主: 大肠杆菌（E. Coli） Recombinant > 90 % pure SDS

VEGF 165 protein
宿主: 大鼠宿主: 大肠杆菌（E. Coli） Recombinant

VEGF 165 protein
宿主: 小鼠宿主: 大肠杆菌（E. Coli） Recombinant

VEGF 165 (Homodimer) (Active) protein
宿主: 人宿主: 大肠杆菌（E. Coli） Recombinant > 95 % by SDS-PAGE Active

说明

Cytokines & Growth Factors

限制

仅限研究用
状态

Lyophilized

溶解方式

The lyophilized VEGF165 should be reconstituted in 50 mM acetic acid to a concentration not lower than 50 μg/mL. For long term storage we recommend to add at least 0.1% human or bovine serum albumin.

缓冲液

50 mM acetic acid

储存条件

-20 °C/-80 °C

储存方法

Lyophilized samples are stable for greater than six months at -20 °C to -70 °C. Reconstituted VEGF165 should be stored in working aliquots at -20 °C.

有效期

6 months
Gaumann, Drexler, Lang, Stoeltzing, Diermeier-Daucher, Buchdunger, Wood, Bold, Breier: "The inhibition of tyrosine kinase receptor signalling in leiomyosarcoma cells using the small molecule kinase inhibitor PTK787/ZK222584 (Vatalanib®)." in: International journal of oncology, Vol. 45, Issue 6, pp. 2267-77, (2014) (PubMed).
抗原

VEGF 165

别名

VEGF165

背景

Human Vascular Endothelial Growth Factor VEGF165, a 23 kDa protein consisting of 165 amino acid residues, is produced as a homodimer. VEGF is a polypeptide growth factor and a member of the platelet-derived growth factor family. It is a specific mitogen for vascular endothelial cells and a strong angiogenic factor in vivo. Two high-affinity tyrosine kinase receptors for VEGF165 have been identified, VEGFR-1 (FLT-1), and VEGFR-2 (KDR). Consistent with the endothelial cell-specific action of VEGF165, expression of both receptor genes has been found predominantly but not exclusively on endothelial cells. Expression of VEGFR-1 was also found on human monocytes, neutrophils (PMNs), bovine brain pericytes and villous and extra villous trophoblast. In addition to its action as a mitogen it is a potent vascular permeability factor (VPF) in vivo. VEGF165 is also a chemo attractant molecule for monocytes and endothelial cells. 5 different proteins are generated by differential splicing: VEGF121, VEGF145, VEGF165, VEGF189 and VEGF206. The most abundant form is VEGF165. Whereas VEGF121 and VEGF165 are secreted proteins, VEGF145, VEGF189 and VEGF206 are strongly cell-associated. The isoforms VEGF145, VEGF165 and VEGF189 bind to heparin with high affinity. VEGF165 is apparently a homo-dimer, but preparations of VEGF165 show some heterogeneity on SDS gels, depending on the secretion of different glycosylation patterns. All dimeric forms have similar biological activities but their bioavailability is very different. There is good evidence that different cells and tissues express different VEGF isoforms. The other members of this increasing growth factor family are VEGF-B, -C, -D and -E. Another member is the Placenta growth factor PlGF.
Synonyms: vascular endothelial growth factor A, VEGFA, VPF, VEGF, MVCD1

分子量

38.2 kDa

NCBI登录号

NM_001171626, NP_001165097

UniProt

P15692