VEGF 165 (Homodimer) (Active) 蛋白
(1 reference)-
- 抗原
- VEGF 165
- 蛋白类型
- Recombinant
- 生物活性
- Active
- 产品特性
- Homodimer
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宿主
- 人
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资源
- Insect Cells
- 原理
- VEGF165
- 序列
- APMAEGGGQN HHEVVKFMDV YQRSYCHPIE TLVDIFQEYP DEIEYIFKPS CVPLMRCGGC CNDEGLECVP TEESNITMQI MRIKPHQGQH IGEMSFLQHN KCECRPKKDR ARQENPCGPC SERRKHLFVQ DPQTCKCSCK NTDSRCKARQ LELNERTCRC DKPRR
- 特异性
- Chromosomal location:6p12
- 产品特性
- Length (aa):165
- 纯度
- > 95 % by SDS-PAGE
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VEGF 165 protein
宿主: 人 宿主: HEK-293 Cells Recombinant > 95% as determined by Tris-Bis PAGE,> 95% as determined by HPLC
VEGF 165 (AA 27-191) protein (His-Avi Tag,Biotin)宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE
VEGF 165 (AA 27-191) (Active) protein (His tag,AVI tag,Biotin)宿主: 人 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Func Active
VEGF 165 (AA 27-191) protein (His-Avi Tag)宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE
VEGF 165 (AA 207-371) protein (His tag)宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 90 % by SDS - PAGE SDS
VEGF 165 protein宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 90 % pure SDS
VEGF 165 protein宿主: 大鼠 宿主: 大肠杆菌（E. Coli） Recombinant
VEGF 165 protein宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant
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- 应用备注
- The ED50 for stimulation of cell proliferation by human umbilical vein endothelial cells for VEGF165 has been determined to be in the range of 1-4 ng/mL.
- 说明
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Cytokines & Growth Factors
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- The lyophilized VEGF165 should be reconstituted in 50 mM acetic acid to a concentration not lower than 50 μg/mL. For long term storage we recommend to add at least 0.1 % human or bovine serum albumin.
- 缓冲液
- 50 mM acetic acid
- 储存条件
- -20 °C,-80 °C
- 储存方法
- Lyophilized samples are stable for greater than six months at -20°C to -70°C. Reconstituted VEGF165 should be stored in working aliquots at -20°C.
- 有效期
- 6 months
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: "ADAM10 and ADAM17 have opposite roles during sprouting angiogenesis." in: Angiogenesis, Vol. 18, Issue 1, pp. 13-22, (2014) (PubMed).
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: "ADAM10 and ADAM17 have opposite roles during sprouting angiogenesis." in: Angiogenesis, Vol. 18, Issue 1, pp. 13-22, (2014) (PubMed).
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- 抗原
- VEGF 165
- 别名
- VEGF165
- 背景
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Human Vascular Endothelial Growth Factor VEGF165, a 23 kDa protein consisting of 165 amino acid residues, is produced as a homodimer. VEGF is a polypeptide growth factor and a member of the platelet-derived growth factor family. It is a specific mitogen for vascular endothelial cells and a strong angiogenic factor in vivo. Two high-affinity tyrosine kinase receptors for VEGF165 have been identified, VEGFR-1 (FLT-1), and VEGFR-2 (KDR). Consistent with the endothelial cell-specific action of VEGF165, expression of both receptor genes has been found predominantly but not exclusively on endothelial cells. Expression of VEGFR-1 was also found on human monocytes, neutrophils (PMNs), bovine brain pericytes and villous and extra villous trophoblast. In addition to its action as a mitogen it is a potent vascular permeability factor (VPF) in vivo. VEGF165 is also a chemo attractant molecule for monocytes and endothelial cells. 5 different proteins are generated by differential splicing: VEGF121, VEGF145, VEGF165, VEGF189 and VEGF206. The most abundant form is VEGF165. Whereas VEGF121 and VEGF165 are secreted proteins, VEGF145, VEGF189 and VEGF206 are strongly cell-associated. The isoforms VEGF145, VEGF165 and VEGF189 bind to heparin with high affinity. VEGF165 is apparently a homo-dimer, but preparations of VEGF165 show some heterogeneity on SDS gels, depending on the secretion of different glycosylation patterns. All dimeric forms have similar biological activities but their bioavailability is very different. There is good evidence that different cells and tissues express different VEGF isoforms. The other members of this increasing growth factor family are VEGF-B, -C, -D and -E. Another member is the Placenta growth factor PlGF
Synonyms: vascular endothelial growth factor A, VEGFA, VPF, VEGF, MVCD1 - 分子量
- 45.0 kDa
- 基因ID
- 7422
- NCBI登录号
- NM_001025366, NP_001020537
- UniProt
- P15692
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