FGF2 蛋白
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- 抗原 See all FGF2 蛋白
- FGF2 (Fibroblast Growth Factor 2 (Basic) (FGF2))
- 蛋白类型
- Recombinant
- 生物活性
- Active
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宿主
- 小鼠
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资源
- 大肠杆菌（E. Coli）
- 原理
- FGF-2 (basic)
- 序列
- ALPEDGGAAF PPGHFKDPKR LYCKNGGFFL RIHPDGRVDG VREKSDPHVK LQLQAEERGV VSIKGVCANR YLAMKEDGRL LASKCVTEEC FFFERLESNN YNTYRSRKYS SWYVALKRTG QYKLGSKTGP GQKAILFLPM SAKS
- 特异性
- Chromosomal location:3 A2-B, 3 19.3 cM
- 产品特性
- Length (aa):144
- 纯度
- > 98 % by SDS-PAGE
- 内毒素水平
- < 0.1 ng per μg of mouse FGF-2
- Top Product
- Discover our top product FGF2 蛋白
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Fibroblast Growth Factor 2 (Basic) (FGF2) (AA 143-288) protein
FGF2 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % as determined by Tris-Bis PAGE
Fibroblast Growth Factor 2 (Basic) (FGF2) (full length) (Active) proteinFGF2 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % as determined by SDS-PAGE CC Active
Fibroblast Growth Factor 2 (Basic) (FGF2) (AA 135-288), (Isoform 1) (Active) proteinFGF2 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % by SDS-PAGE analysis. Active
Fibroblast Growth Factor 2 (Basic) (FGF2) (AA 143-288) (Active) protein (AVI tag,His tag,Biotin)FGF2 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant >90 % as determined by SDS-PAGE. Active
Fibroblast Growth Factor 2 (Basic) (FGF2) (Active) protein (His tag)FGF2 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % as determined by SDS-PAGE Active
Fibroblast Growth Factor 2 (Basic) (FGF2) (AA 9-154) protein (His tag)FGF2 宿主: 大鼠 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Fibroblast Growth Factor 2 (Basic) (FGF2) (AA 189-288) protein (GST tag)FGF2 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
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- 应用备注
- The ED50 for stimulation of cell proliferation by human umbilical vein endothelial cells for mouse FGF-2 has been determined to be in the range of 0.1-2 ng/mL.
- 说明
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Cytokines & Growth Factors
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- The lyophilized basic FGF should be reconstituted in water containing at least 0.1 % human or bovine serum albumin to a concentration not lower than 10 μg/mL.
- 缓冲液
- PBS
- 储存条件
- 4 °C,-20 °C,-80 °C
- 储存方法
- Lyophilized samples are stable for greater than six months at -20°C to -70°C. Basic FGF can be stored in high-salt buffer (PBS, 1M NaCl) at 4°C for 2-4 weeks.
- 有效期
- 6 months
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- 抗原
- FGF2 (Fibroblast Growth Factor 2 (Basic) (FGF2))
- 别名
- FGF-2 (FGF2 产品)
- 别名
- BFGF Protein, FGF-2 Protein, FGFB Protein, HBGF-2 Protein, Fgf-2 Protein, Fgfb Protein, bFGF Protein, fibroblast growth factor 2 Protein, fibroblast growth factor 2 (basic) Protein, FGF2 Protein, Fgf2 Protein, fgf2 Protein
- 背景
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The FGF family is composed of at least 23 polypeptides that show a variety of biological activities towards cells of mesenchymal, neuronal and epithelial origin. All members are heparin-binding growth factors (HB-GF). Until the structure of basic fibroblast growth factor (bFGF/FGF-2) was determined, a number of synonyms was used to describe this growth factor. As is often the case, the nomenclature reflected the observed activities reported by individual groups. Basic FGF has been reported as leukemia growth factor, macrophage growth factor, endothelial growth factor and tumor angiogenesis factor. The eventual isolation and characterization of bFGF was done from soluble brain extracts. bFGF was found to have a molecular mass of 16.5 kDa and to be 154 amino acids in length. Interestingly, bFGF contains no hydrophobic leader sequence previously thought to be required for cell secretion. Basic FGF bears 55 % homology to acidic FGF and also seems to exist in three forms: the 154 amino-acid form and two other truncated versions of 146 and 131 amino acids lacking the N-terminal 9 and 24 residues. Acidic and basic FGF compete for the binding to 125 kDa and 145 kDa receptor species. However, acidic FGF has a higher affinity for the 125 kDa species, while basic FGF has a higher affinity for the 145 kDa species. FGF receptor activation leads to the activation of MAP kinase and protein kinase C. FGF’s induce the proliferative response in cells derived from mesoderm and neuroectoderm. It seems that basic FGF reduces the average doubling time by shortening the G1 phase of the cell cycle. Furthermore, it has been reported to induce the release of plasminogen activator by endothelial cells. Perhaps one of the most potentially significant applications of bFGF is related to its reported ability to induce angiogenesis.
Synonyms: Fgf2, Fgfb, bFGF, Fgf-2 - 分子量
- 16.35 kDa
- 基因ID
- 14173
- NCBI登录号
- NM_008006, NP_032032
- UniProt
- P15655
- 途径
- RTK signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, C21-Steroid Hormone Metabolic Process, Inositol Metabolic Process, Glycosaminoglycan Metabolic Process, Protein targeting to Nucleus, S100 Proteins
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