PDGF-AB Heterodimer (Homodimer) (Active) 蛋白
宿主: 人
宿主: 大肠杆菌（E. Coli）
Recombinant
> 95 % by SDS-PAGE
Active
-
- 抗原
- PDGF-AB Heterodimer
- 蛋白类型
- Recombinant
- 生物活性
- Active
- 产品特性
- Homodimer
- 宿主
- 人
-
资源
- 大肠杆菌（E. Coli）
- 原理
- PDGF-AB
- 序列
- Alpha chain: MSIEEAVPAV CKTRTVIYEI PRSQVDPTSA NFLIWPPCVE VKRCTGCCNT SSVKCQPSRV HHRSVKVAKV EYVRKKPKLK EVQVRLEEHL ECACATTSLN PDYREEDTGR PRESGKKRKR KRLKPT Beta chain: MSLGSLTIAE PAMIAECKTR TEVFEISRRL IDRTNANFLV WPPCVEVQRC SGCCNNRNVQ CRPTQVQLRP VQVRKIGIVR KKPIFKKATV TLGDHLACKC ETVAAARPVT
- 特异性
- Chromosomal location:7p22/22q13.1
- 产品特性
- Length (aa):126/110
- 纯度
- > 95 % by SDS-PAGE
- 内毒素水平
- < 0.1 ng per ug of PDGF-AB
-
PDGF-AB Heterodimer protein
宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant
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- 应用备注
- The biological activity was determined by the induction of proliferation in NHDF cells (Normal Human Dermal Fibroblasts).
- 说明
-
Cytokines & Growth Factors
- 限制
- 仅限研究用
-
- 状态
- Lyophilized
- 溶解方式
- Centrifuge vial prior to opening. The lyophilized PDGF-AB should be reconstituted in 50 mM acetic acid to a concentration not lower than 100 μg/mL. For long term storage of reconstituted protein addition of carrier protein (e.g. BSA or HSA, 0.1 % ) is recommended.
- 缓冲液
- 50 mM acetic acid
- 注意事项
- Centrifuge vial prior to opening. Avoid repeated freeze-thaw cycles.
- 储存条件
- RT,-20 °C,-80 °C
- 储存方法
- The lyophilized PDGF-AB is stable for a few weeks at room temperature, but best stored at -20°C. Reconstituted PDGF-AB is best stored at -20°C to -70°C. Avoid repeated freeze-thaw cycles.
-
- 抗原
- PDGF-AB Heterodimer
- 别名
- PDGF-AB
- 别名
- PDGF-A Protein, PDGF1 Protein, platelet derived growth factor subunit A Protein, PDGFA Protein
- 背景
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PDGFs are disulfide-linked dimers consisting of two 12.0-13.5 kDa polypeptide chains, designated PDGF-A and PDGF-B chains. The three naturally occurring PDGFs, PDGF-AA, PDGF-BB and PDGF-AB, are potent mitogens for a variety of cell types including smooth muscle cells, connective tissue cells, bone and cartilage cells, and some blood cells. The PDGFs are stored in platelet α-granules and are released upon platelet activation. The PDGFs are involved in a number of biological processes, including hyperplasia, chemotaxis, embryonic neuron development, and respiratory tubule epithelial cell development. Two distinct signaling receptors used by PDGFs have been identified and named PDGFR-α and PDGFR-β. PDGFR-α is high-affinity receptor for each of the three PDGF forms. On the other hand, PDGFR-β interacts with only PDGF-BB and PDGF-AB. Recombinant human PDGF-AB is a 25.5 kDa disulfide-linked dimer, consisting of one A chain and one B chains (234 total amino acids).
Synonyms: PDGFA, PDGF1, PDGF-A - 分子量
- 25.5 kDa
- 基因ID
- 5154, 5155
- NCBI登录号
- NM_002608, NM_002607, NP_002599, NP_002598
- UniProt
- P04085, P01127
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