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CNTF 蛋白

CNTF 宿主: 大鼠 宿主: 大肠杆菌(E. Coli) Recombinant > 98 % by SDS-PAGE Active
产品编号 ABIN1589602
发货至: 中国
  • 抗原 See all CNTF 蛋白
    CNTF (Ciliary Neurotrophic Factor (CNTF))
    蛋白类型
    Recombinant
    生物活性
    Active
    宿主
    • 19
    • 7
    • 4
    • 2
    • 2
    • 2
    大鼠
    资源
    • 25
    • 3
    • 3
    • 2
    • 1
    大肠杆菌(E. Coli)
    原理
    CNTF
    序列
    AFAEQTPLTL HRRDLCSRSI WLARKIRSDL TALMESYVKH QGLNKNINLD SVDGVPVAST DRWSEMTEAE RLQENLQAYR TFQGMLTKLL EDQRVHFTPT EGDFHQAIHT LMLQVSAFAY QLEELMVLLE QKIPENEADG MPATVGDGGL FEKKLWGLKV LQELSQWTVR SIHDLRVISS HQMGISALES HYGAKDKQM
    特异性
    Chromosomal location:1q43
    产品特性
    Length (aa):199
    纯度
    > 98 % by SDS-PAGE
    内毒素水平
    < 0.1 ng per μg of CNTF
    Top Product
    Discover our top product CNTF 蛋白
  • 应用备注
    Measured in a cell proliferation assay using TF1 human erythroleukemic cells [Kitamura T et al, J Cell Physiol 140, 1989). The ED50 for this effect is typically 3 - 15 ng/mL.
    说明

    Cytokines & Growth Factors

    限制
    仅限研究用
  • 状态
    Lyophilized
    溶解方式
    Rat CNTF should be reconstituted in PBS to a concentration of 0.1 mg/mL. This solution can be diluted in water or other buffered solutions.
    缓冲液
    5 mM sodium acetate, pH 6.5
    储存条件
    RT,-20 °C
    储存方法
    The lyophilized powder although stable at room temperature for 3 weeks, is best stored desiccated at -20°C. Reconstituted rat CNTF should be stored in working aliquots at -20°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
  • 抗原
    CNTF (Ciliary Neurotrophic Factor (CNTF))
    别名
    CNTF (CNTF 产品)
    背景
    Ciliary neurotrophic factor (CNTF) is a polypeptide initially purified from chick embryo ocular tissue and identified as a trophic factor for embryonic chick ciliary parasympathetic neurons in culture. Subsequent studies have demonstrated that CNTF is a survival factor for additional neuronal cell types including: primary sensory neurons, motor neurons, basal forebrain neurons and type 2 astrocytes. CNTF has also been shown to prevent the degeneration of motor axons after axotomy. The cDNA for CNTF encodes a 200 amino acid residue polypeptide that lacks a signal sequence. CNTF is highly conserved across species and exhibits cross-species activities. Human and rat CNTF share approximately 83% homology in their protein sequence. CNTF is structurally related to IL6, IL11, LIF, and OSM. All of these four helix bundle cytokines share gp130 as a signal-transducing subunit in their receptor complexes. The cDNA for recombinant rat CNTF (Ala2–Met200) was cloned from total RNA of a rat embryo using standard protocols. Ciliary Neurotrophic Factor (CNTF) is a potent neural factor that was originally characterized as a survivability factor for chick ciliary neurons in vitro. More recently, CNTF has been shown to promote survivability and differentiation of other neuronal cell types. Rat CNTF is a 22.7 kDa protein containing 199 amino acid residues.
    Synonyms: Cntf
    分子量
    22.7 kDa
    基因ID
    25707
    NCBI登录号
    NM_013166, NP_037298
    UniProt
    P20294
    途径
    JAK/STAT Signaling
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