PDGFC Protein (Homodimer)
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- 抗原 See all PDGFC 蛋白
- PDGFC (Platelet-Derived Growth Factor C (PDGFC))
- 蛋白类型
- Recombinant
- 生物活性
- Active
- 产品特性
- Homodimer
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宿主
- Parapoxvirus
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资源
- 大肠杆菌（E. Coli）
- 原理
- VEGF-E
- 序列
- MGSSHHHHHH SSGLVPRGSH DSTKTWSEVF ENSGCKPRPM VFRVHDEHPE LTSQRFNPPC VTLMRCGGCC NDESLECVPT EEANVTMQLM GASVSGGNGM QHLSFVEHKK CDCKPPLTTT PPTTTRPPRR RR
- 产品特性
- Length (aa):132
- 纯度
- > 90 % by SDS-PAGE
- 内毒素水平
- < 0.1 ng per μg of ov-VEGF-E
- Top Product
- Discover our top product PDGFC 蛋白
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Platelet-Derived Growth Factor C (PDGFC) (AA 236-345) protein (GST tag)
PDGFC 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Platelet-Derived Growth Factor C (PDGFC) (AA 1-345) protein (GST tag)PDGFC 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Platelet-Derived Growth Factor C (PDGFC) proteinPDGFC 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % WB, SDS, ELISA, IP
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- 应用备注
- Measured in a cell proliferation assay using primary HUVECs. The ED50 for this effect is typically 1 - 5 ng/mL.
- 说明
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Cytokines & Growth Factors
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- The lyophilized ovVEGF-E should be reconstituted in water or medium to a concentration not lower than 50 μg/mL. For long term storage we would recommend to add at least 0.1 % human or bovine serum albumin.
- 缓冲液
- PBS
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- 抗原
- PDGFC (Platelet-Derived Growth Factor C (PDGFC))
- 别名
- VEGF-E (PDGFC 产品)
- 别名
- SCDGF Protein, FALLOTEIN Protein, 1110064L01Rik Protein, AI647969 Protein, PDGF-C Protein, platelet derived growth factor C Protein, platelet-derived growth factor, C polypeptide Protein, PDGFC Protein, Pdgfc Protein
- 物质类
- Viral Protein
- 背景
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A DNA sequence encoding the mature variant of ovVEGF-E isolate D1701 (Dehio et al., 1999, GenBank accession No. AF106020) was expressed in E. coli as a 132 amino acid residue fusion protein with an N-terminal His-tag sequence and a thrombin cleavage site. Recombinant VEGF-E homodimer was dimerized in vitro and has a predicted mass of approximately 35 kDa. Based on sequence similarity to VEGF-A, a gene encoding a VEGF homologue has recently been discovered in the genome of Orf virus (OV) (Lyttle et al., 1994). Different isolates of Orf virus show significant amino acid sequence similarity to VEGF-A and described as a viral virulence factor that appears to be derived from captured host genes. All eight cysteine residues of the central cysteine knot motif characteristic of members of the VEGF family are conserved among other residues in the VEGF-E proteins (Dehio et al., 1999, Wise et al., 1999). Alignment of all mammalian VEGF sequences indicated that VEGF-E is distinct from the previously described VEGFs but most closely related to VEGF-A. Like VEGF-A, VEGF-E was found to bind with high affinity to VEGF receptor-2 (KDR) resulting in receptor autophosphorylation, whilst in contrast to VEGF-A, VEGF-E can not bind to VEGF receptor-1 (Flt-1). Furthermore VEGF-E can also not bind to VEGF receptor-3 (FLT-4). Therefore VEGF-E is a potent angiogenic factor selectively binding to VEGF receptor –2/KDR.
Synonyms: Vascular Endothelial Growth Factor-E - 分子量
- 35 kDa (Dimer)
- UniProt
- Q9YMF3
- 途径
- RTK signaling, Platelet-derived growth Factor Receptor Signaling
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