VEGF189 Protein (Homodimer)
宿主: 人
宿主: 大肠杆菌（E. Coli）
Recombinant
> 98 % by SDS-PAGE
Active
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- 抗原 See all VEGF189 products
- VEGF189 (Vascular Endothelial Growth Factor 189 (VEGF189))
- 蛋白类型
- Recombinant
- 生物活性
- Active
- 产品特性
- Homodimer
- 宿主
- 人
- 资源
- 大肠杆菌（E. Coli）
- 原理
- VEGF189
- 序列
- APMAEGGGQN HHEVVKFMDV YQRSYCHPIE TLVDIFQEYP DEIEYIFKPS CVPLMRCGGC CNDEGLECVP TEESNITMQI MRIKPHQGQH IGEMSFLQHN KCECRPKKDR ARQEKKSVRG KGKGQKRKRK KSRYKSWSVP CGPCSERRKH LFVQDPQTCK CSCKNTDSRC KARQLELNER TCRCDKPRR
- 特异性
- Chromosomal location:6p12
- 产品特性
- Length (aa):189
- 纯度
- > 98 % by SDS-PAGE
- 内毒素水平
- < 0.1 ng per μg of human VEGF189
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- 应用备注
- Measured by the stimulation of cell proliferation in human umbilical vein endothelial cells in the range of 2-20 ng/mL.
- 说明
-
Cytokines & Growth Factors
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- The lyophilized VEGF189 is soluble in water and most aqueous buffers. The lyophilized VEGF189 should be reconstituted in PBS or medium containing at least 0.1 % human or bovine serum albumin to a concentration not lower than 50 μg/mL.
- 缓冲液
- 50 mM acetic acid
- 注意事项
- Avoid repeated freeze-thaw cycles.
- 储存条件
- RT,-20 °C
- 储存方法
- The lyophilized protein is stable for a few weeks at room temperature, but best stored at -20°C. Reconstituted VEGF189 should be stored in working aliquots at -20°C. Avoid repeated freeze-thaw cycles.
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- 抗原
- VEGF189 (Vascular Endothelial Growth Factor 189 (VEGF189))
- Abstract
- VEGF189 产品
- 背景
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Human Vascular Endothelial Growth Factor VEGF189, a 21 kDa protein consisting of 189 amino acid residues, is produced as a homodimer. VEGF is a polypeptide growth factor and a member of the platelet-derived growth factor family. It is a specific mitogen for vascular endothelial cells and a strong angiogenic factor in vivo. Two high-affinity tyrosine kinase receptors for VEGF165 have been identified, VEGFR-1 (FLT-1), and VEGFR-2 (KDR). Consistent with the endothelial cell-specific action of VEGF165, expression of both receptor genes has been found predominantly but not exclusively on endothelial cells. Expression of VEGFR-1 was also found on human monocytes, neutrophils (PMNs), bovine brain pericytes and villous and extra villous trophoblast. In addition to its action as a mitogen it is a potent vasular permeability factor (VPF) in vivo. VEGF165 is also a chemo attractant molecule for monocytes and endothelial cells. 5 different proteins are generated by diffential splicing: VEGF121, VEGF145, VEGF165, VEGF189 and VEGF206. The most abundant form is VEGF165. Where¬as VEGF121 and VEGF165 are secreted proteins, VEGF145, VEGF189 and VEGF206 are strongly cell-associated. The isoforms VEGF145, VEGF165 and VEGF189 bind to heparin with high affinity. VEGF165 is appa¬rently a homo-dimer, but preparations of VEGF165 show some heterogeneity on SDS gels, depending on the secretion of different glycosylation patterns. All dimeric forms have similar biological activities but their bio¬availability is very different. There is good evidence that heterodimeric molecules between the different isoforms also exists and that different cells and tissues express different VEGF isoforms. The other members of this in¬creasing growth factor family are VEGF-B, -C, -D and -E. Another member is the Placenta growth factor PlGF.
Synonyms: vascular endothelial growth factor A, VEGFA,VPF, VEGF, MVCD1 - 分子量
- ~ 42.0 kDa
- 基因ID
- 7422
- NCBI登录号
- NM_001025366, NP_001020537
- UniProt
- P15692
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