(4 references)-
- 抗原 See all Protein L 蛋白
- Protein L
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宿主
- Peptostreptococcus magnus
- 宿主
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- 标记
- This Protein L protein is labelled with Biotin.
- 应用范围
- Western Blotting (WB), Dot Blot (DB), Immunohistochemistry (IHC), ELISA
- Top Product
- Discover our top product Protein L 蛋白
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Protein L (AA 106-470) (Active) protein (His tag,AVI tag,Biotin)
宿主: Peptostreptococcus magnus 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS-PAGE. Active
Protein L protein (His tag)宿主: Peptostreptococcus magnus 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS-PAGE.
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- 应用备注
- Working concentrations for specific applications should be determined by titration assay. The chart below may serve as a useful guideline, but primary antibody, temperature, incubation time, the amount of antigen, and the sensitivity of the detection methods can all affect the ideal working concentration. Dot blot: 1:2,000-1:20,000 Western blot: 1:2,000-1:20,000 ELISA: 1:20,000-1:100,000 Other: user-optimized
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Reconstitute the lyophilized product with deionized water (or equivalent) to the final concentration of 1.0 mg/ml.
- 浓度
- 1 mg/ml
- 缓冲液
- PBS (pH 7.4) containing 10 mg/ml BSA and 0.02% sodium azide
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- -20 °C
- 储存方法
- The product is stable in lyophilized form if stored at -20°C or below. The reconstituted product can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
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: "Systemic treatment with CAR-engineered T cells against PSCA delays subcutaneous tumor growth and prolongs survival of mice." in: BMC cancer, Vol. 14, pp. 30, (2014) (PubMed).
: "A novel chimeric antigen receptor against prostate stem cell antigen mediates tumor destruction in a humanized mouse model of pancreatic cancer." in: Human gene therapy, Vol. 25, Issue 12, pp. 1003-12, (2014) (PubMed).
: "Redirected antitumor activity of primary human lymphocytes transduced with a fully human anti-mesothelin chimeric receptor." in: Molecular therapy : the journal of the American Society of Gene Therapy, Vol. 20, Issue 3, pp. 633-43, (2012) (PubMed).
: "Recognition of glioma stem cells by genetically modified T cells targeting EGFRvIII and development of adoptive cell therapy for glioma." in: Human gene therapy, Vol. 23, Issue 10, pp. 1043-53, (2012) (PubMed).
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: "Systemic treatment with CAR-engineered T cells against PSCA delays subcutaneous tumor growth and prolongs survival of mice." in: BMC cancer, Vol. 14, pp. 30, (2014) (PubMed).
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- 抗原
- Protein L
- Abstract
- Protein L 产品
- 背景
- Protein L is a cell surface protein from Peptostreptoccocus magnus that binds to the variable light chains (kappa chain) of immunoglobulins without interfering with antigen binding. In contrast to IgG-binding proteins, such as protein A and protein G, protein L can be used for the detection and purification of mammalian kappa light chain antibodies of all classes. Biotin-Protein L, which is produced by covalently linking biotin to protein L, can be used in immunodetections, such as immunoblotting (western or dot blot), ELISA, and immunohistochemistry.
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