Protein L Protein (HRP)
This Protein L protein is expressed in .
Quick Overview for Protein L Protein (HRP) (ABIN1536529)
抗原
See all Protein L 蛋白
Protein L
宿主
Peptostreptococcus magnus
宿主
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应用范围
Western Blotting (WB), Dot Blot (DB), Immunohistochemistry (IHC), ELISA
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标记
- This Protein L protein is labelled with HRP.
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Protein L protein (His tag)
宿主: Peptostreptococcus magnus 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS-PAGE.
Protein L (AA 106-470) (Active) protein (His tag,AVI tag,Biotin)宿主: Peptostreptococcus magnus 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS-PAGE. Active
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应用备注
- The ideal working concentrations for each specific application should be determined by titration assay. Appropriate concentrations will be affected by several factors including primary antibody, antigen amount, sensitivity of detection method, temperature, and incubation time. The following dilution ranges are recommended starting points: Dot blot: 1:2,000-1:20,000 Western blot: 1:2,000-1:20,000 ELISA: 1:20,000-1:200,000 Other applications: user-optimized
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限制
- 仅限研究用
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浓度
- 1 mg/ml
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缓冲液
- PBS (pH 7.4) containing 10 mg/ml BSA and 0.02% sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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储存方法
- The reagent may be stored at 4°C for short periods of up to one-month. For longer periods, aliquot and store at the reagent at -20°C for longer storage. Avoid repeated freeze/thaw cycles. If slight precipitation occurs upon prolonged storage, clarify the solution by centrifugation before use.
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- Protein L
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背景
- Protein L is a cell surface protein expressed by Peptostreptoccocus magnus, that binds to the variable light chains (kappa chains) of immunoglobulins without interfering with antigen binding. In contrast to IgG-binding proteins such as protein A and protein G, protein L can be used for the detection and purification of mammalian kappa light chain antibodies of all classes. HRP-Protein L is produced by covalently linking HRP to protein L. The complex can be used in immuno-detection such as immunoblotting (western or dot blot), ELISA, and immunohistochemistry.
抗原
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