MANF 蛋白
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- 抗原 See all MANF 蛋白
- MANF (Mesencephalic Astrocyte-Derived Neurotrophic Factor (MANF))
- 蛋白类型
- Recombinant
- 生物活性
- Active
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宿主
- 人
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资源
- CHO Cells
- 应用范围
- Western Blotting (WB), SDS-PAGE (SDS), Cell Culture (CC), In vivo Studies (in vivo)
- 产品特性
- Bio-activity testing: Protein is biologically active. Suitable for cell culture and in vivo testing. Human recombinant MANF has been tested in rat &-OHDA model of Parkinson's disease and shown neureprotection and neurorestoration. MANF has also shown neuroprotection in animal models of stroke
- 纯化方法
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Purified by ion-exchange chromatography and gel-filtration from serum-free CHO growth media.
Purified Protein is characterized as homogenous material by mass-spectroscopy. - 内毒素水平
- Less than 1EU/µg of protein as determined by LAL method
- Top Product
- Discover our top product MANF 蛋白
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Mesencephalic Astrocyte-Derived Neurotrophic Factor (MANF) (Active) protein
MANF 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS-PAGE Func Active
Mesencephalic Astrocyte-Derived Neurotrophic Factor (MANF) (AA 25-182) protein (His tag)MANF 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Mesencephalic Astrocyte-Derived Neurotrophic Factor (MANF) (AA 1-182) protein (GST tag)MANF 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Mesencephalic Astrocyte-Derived Neurotrophic Factor (MANF) protein (His tag)MANF 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 90 % as determined by SDS-PAGE
Mesencephalic Astrocyte-Derived Neurotrophic Factor (MANF) protein (Myc-DYKDDDDK Tag)MANF 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- 说明
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Synonyms: Mesencephalic astrocyte-derived neurotrophic factor - MANF, ARMET, ARP
- 限制
- 仅限研究用
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- 浓度
- 1 mg/ml Concentration of the protein is determined by BCA Protein Analysis kit (Pierce). BSA was used as a standard
- 缓冲液
- PBS pH 7.4
- 注意事项
- Recommended to aliquot into smaller quantities. Avoid repeated freeze-thaw cycles.
- 储存条件
- -80 °C
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- 抗原
- MANF (Mesencephalic Astrocyte-Derived Neurotrophic Factor (MANF))
- 别名
- MANF (MANF 产品)
- 背景
- MANF is a trophic factor for midbrain dopamine neurons in vivo. It prevents the 6-OHDA- induced degeneration of dopamine neurons in rodent models of Parkinson’s disease (Lindholm et al., 2008, Voutilainen et al., 2009). When administered after 6-OHDA-lesioning it restores the dopaminergic function and prevents degeneration of dopamine neurons in substantia nigra pars compacta.
- 分子量
- Approximately 18.1 kDa, a single non-glycosylated polypeptide chain containing 163 amino acids.
- UniProt
- P55145
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