CDNF 蛋白
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- 抗原 See all CDNF 蛋白
- CDNF (Cerebral Dopamine Neurotrophic Factor (CDNF))
- 蛋白类型
- Recombinant
- 生物活性
- Active
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宿主
- 人
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资源
- CHO Cells
- 应用范围
- Western Blotting (WB), SDS-PAGE (SDS), Cell Culture (CC), In vivo Studies (in vivo)
- 产品特性
- Biological properties: Protein is biologically active. Suitable for cell culture and in vivo testing. Biological activity of human CDNF was tested in rat 6-OHDA model of Parkinson’s disease and shown to be both neuroprotective and neurorestorative (for methods see: Lindholm et al. 2007, Voutilainen et al. 2011)
- 纯化方法
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Purified by ion-exchange chromatography and gel-filtration from serum-free CHO growth media.
Protein is sterile-filtrated through 0.22 µg filter.
Purified human CDNF is characterized as homogenous material by mass-spectroscopy. - 内毒素水平
- Less than 1EU/µg of protein as determined by LAL method
- Top Product
- Discover our top product CDNF 蛋白
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Cerebral Dopamine Neurotrophic Factor (CDNF) (AA 25-187) protein (His tag)
CDNF 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Cerebral Dopamine Neurotrophic Factor (CDNF) protein (His tag)CDNF 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by SDS-PAGE
Cerebral Dopamine Neurotrophic Factor (CDNF) proteinCDNF 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % WB, SDS, ELISA, IP
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- 说明
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Synonyms: Cerebral dopamine neurotrophic factor - CDNF, ARMETL1.
- 限制
- 仅限研究用
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- 浓度
- 1 mg/ml Concentration of the protein is determined by BCA Protein Analysis kit (Pierce). BSA was used as a standard
- 缓冲液
- PBS pH 7.5
- 注意事项
- Recommended to aliquot into smaller quantities. Avoid repeated freeze-thaw cycles.
- 储存条件
- -80 °C
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- 抗原
- CDNF (Cerebral Dopamine Neurotrophic Factor (CDNF))
- 别名
- CDNF (CDNF 产品)
- 别名
- ARMETL1 Protein, 9330140G23 Protein, Armetl1 Protein, cerebral dopamine neurotrophic factor Protein, CDNF Protein, Cdnf Protein
- 背景
- CDNF is a trophic factor for midbrain dopamine neurons in vivo. It prevents the 6-OHDA- (Lindholm et al. 20007, Voutilainen et al., 2011) and MPTP-induced degeneration (Airavaara et al., 2012) of dopamine neurons in rodent models of Parkinson’s disease. When administered after 6-OHDA or MPTP –lesioning it restores the dopaminergic function and prevents degeneration of dopamine neurons in substantia nigra pars compacta.
- 分子量
- Approximately 18.5 kDa, a single non-glycosylated polypeptide chain containing 163 amino acids.
- UniProt
- Q49AH0
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