CD40 Ligand Protein (CD40LG) (AA 18-111) (DYKDDDDK Tag)
-
- 抗原 See all CD40 Ligand (CD40LG) 蛋白
- CD40 Ligand (CD40LG)
- 蛋白类型
- Recombinant
- 生物活性
- Active
- 产品特性
- AA 18-111
-
宿主
- 人, 小鼠
-
资源
- CHO Cells
- 标记
- This CD40 Ligand protein is labelled with DYKDDDDK Tag.
- 应用范围
- SDS-PAGE (SDS)
- 品牌
- MegaCD40L™
- 特异性
- Binds to human and mouse CD40.
- 交叉反应
- 人, 小鼠
- 产品特性
- Mouse CD40L (aa 115-260) is fused at the N-terminus to mouse ACRP30headless (aa 18-111) and a FLAG®-tag.
- 纯度
- >95 % (SDS-PAGE)
- 内毒素水平
- <0.01EU/μg purified protein (LAL test, Lonza).
- Top Product
- Discover our top product CD40LG 蛋白
-
-
- 应用备注
- Optimal working dilution should be determined by the investigator.
- 说明
-
Induces B cells activation (as demonstrated by dose-dependent upregulation of CD86).
- 限制
- 仅限研究用
-
- 状态
- Lyophilized
- 浓度
- Lot specific
- 缓冲液
- Lyophilized. Contains PBS.
- 储存条件
- 4 °C,-20 °C
- 储存方法
-
Short Term Storage: +4°C
Long Term Storage: -20°C
Stable for at least 6 months after receipt when stored at -20°C. - 有效期
- 6 months
-
-
T cells dampen innate immune responses through inhibition of NLRP1 and NLRP3 inflammasomes." in: Nature, Vol. 460, Issue 7252, pp. 269-73, (2009) (PubMed).
: "Two adjacent trimeric Fas ligands are required for Fas signaling and formation of a death-inducing signaling complex." in: Molecular and cellular biology, Vol. 23, Issue 4, pp. 1428-40, (2003) (PubMed).
: "
-
T cells dampen innate immune responses through inhibition of NLRP1 and NLRP3 inflammasomes." in: Nature, Vol. 460, Issue 7252, pp. 269-73, (2009) (PubMed).
-
- 抗原
- CD40 Ligand (CD40LG)
- 别名
- MegaCD40L (CD40LG 产品)
- 别名
- CD154 Protein, cd40l Protein, CD40L Protein, HIGM1 Protein, IGM Protein, IMD3 Protein, T-BAM Protein, TNFSF5 Protein, TRAP Protein, gp39 Protein, hCD40L Protein, CD40-L Protein, Cd40l Protein, Ly-62 Protein, Ly62 Protein, Tnfsf5 Protein, CD40 ligand Protein, TNF superfamily member 5 Protein, CD40LG Protein, cd40l Protein, Cd40lg Protein
- 背景
- MultimericCD40L™ is a high activity construct in which two trimeric CD40 ligands are artificially linked via the collagen domain of ACRP30. This construct very effectively simulates the natural membrane-assisted aggregation of CD40L in vivo. It provides a simple and equally potent alternative to CD40L+enhancer combinations. MultimericCD40L™ has shown to suppress alum-induced IL-1beta release and caspase-1 activation in a dose-, CD40- and time dependent manner, without affecting BMDM (Bone marrow-derived macrophages) viability. It also effectively suppressed the inflammasome function triggered by NLRP3 activators. The secretion of caspase-1 independent inflammatory mediators has been shown to be unaltered or even enhanced.
- 分子量
- ~35-40kDa
- UniProt
- P27548
- 途径
- NF-kappaB Signaling, Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints
-