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- 抗原 See all CXCL10 蛋白
- CXCL10 (Chemokine (C-X-C Motif) Ligand 10 (CXCL10))
- 蛋白类型
- Recombinant
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宿主
- 人
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资源
- 大肠杆菌(E. Coli)
- 应用范围
- ELISA
- 品牌
- BD Pharmingen™
- 特异性
- Biological Activity: This preparation of recombinant human IP-10 protein has not been evaluated for biological activity. Carrier proteins should be pre-screened for possible effects in an appropriate experimental system. Carrier proteins may effect experimental results due to toxicity, high endotoxin levels or possible blocking activity.
- 产品特性
- The recombinant human IP-10 was produced and found to be ≥ 95% pure by SDS-PAGE analysis and visualized by silver strain.
- 纯化方法
- The recombinant human IP-10 was produced and found to be ≥ 95 % pure by SDS-PAGE analysis and visualized by silver strain.
- 纯度
- ≥ 95 %
- 过滤
- 0.22 μm filtered
- Top Product
- Discover our top product CXCL10 蛋白
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- 应用备注
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ELISA Standard: The recombinant human IP-10 (Cat. No. 551130) is useful as a quantitative standard for a sandwich ELISA for measuring human IP-10 protein levels. Purified 4D5 antibody (Cat. No. 555046) can be paired with the biotinylated 6D4 antibody (Cat. No. 555048) with recombinant human IP-10 as the standard. To obtain linear standard curves, doubling dilutions of human IP-10 ranging from ~1,000 to 5 pg/ml are recommended for inclusion in each ELISA plate.
Note: This ELISA pair is recommended primarily for measuring cytokine from experimental cell culture systems. These ELISA reagents are not recommended for assaying serum or plasma samples. For measuring human-IP-10 in serum or plasma our BD OptEIA™ Human IP-10 Set (Cat. No. 550926) is specially formulated and recommended. - 说明
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BD Pharmingen™ Recombinant Human IP-10
- 限制
- 仅限研究用
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- 储存液
- Azide free
- 注意事项
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This preparation contains no preservatives, thus it should be handled under aseptic conditions. Recombinant human IP-10 is supplied lyophilized from a solution of aqueous buffered solution containing 50 µg BSA per 1 µg of cytokine as carrier protein. No sodium azide has been added to the preparation and the endotoxin level is less than 1 EU per 1 µg of the cytokine as determined by the LAL method. The product can be reconstituted in sterile neutral buffer containing not less than 0.5 - 10.0 mg/ml carrier protein such as human or bovine serum albumin, aliquoted into polypropylene microtubes and stored at -80°C.
Note: Failure to add carrier protein or store at indicated temperatures may result in a loss of activity. - 储存条件
- -80 °C
- 储存方法
- This preparation contains no preservatives, thus it should be handled under aseptic conditions. Store product at -80°C prior to use or for long term storage of stock solutions. Avoid multiple freeze-thaws of product. Recombinant human IP-10 is supplied lyophilized from a solution of aqueous buffered solution containing 50 μg BSA per 1 μg of cytokine as carrier protein. No sodium azide has been added to the preparation and the endotoxin level is less than 1 EU per 1 μg of the cytokine as determined by the LAL method. The product can be reconstituted in sterile neutral buffer containing not less than 0.5 - 10.0 mg/ml carrier protein such as human or bovine serum albumin, aliquoted into polypropylene microtubes and stored at -80°C. NOTE: Failure to add carrier protein** or store at indicated temperatures may result in a loss of activity.
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Mig and IP-10: CXC chemokines that target lymphocytes." in: Journal of leukocyte biology, Vol. 61, Issue 3, pp. 246-57, (1997) (PubMed).
: "Chemokine receptor specific for IP10 and mig: structure, function, and expression in activated T-lymphocytes." in: The Journal of experimental medicine, Vol. 184, Issue 3, pp. 963-9, (1997) (PubMed).
: "Biochemical characterization of a gamma interferon-inducible cytokine (IP-10)." in: The Journal of experimental medicine, Vol. 166, Issue 4, pp. 1084-97, (1987) (PubMed).
: "Interferon-inducible gene maps to a chromosomal band associated with a (4;11) translocation in acute leukemia cells." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 84, Issue 9, pp. 2868-71, (1987) (PubMed).
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Mig and IP-10: CXC chemokines that target lymphocytes." in: Journal of leukocyte biology, Vol. 61, Issue 3, pp. 246-57, (1997) (PubMed).
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- 抗原
- CXCL10 (Chemokine (C-X-C Motif) Ligand 10 (CXCL10))
- 别名
- IP-10 (CXCL10 产品)
- 别名
- CXCL10 Protein, C7 Protein, IFI10 Protein, INP10 Protein, IP-10 Protein, SCYB10 Protein, crg-2 Protein, gIP-10 Protein, mob-1 Protein, CRG-2 Protein, IP10 Protein, Ifi10 Protein, Scyb10 Protein, C-X-C motif chemokine ligand 10 Protein, C-X-C motif chemokine 10 Protein, chemokine (C-X-C motif) ligand 10 Protein, CXCL10 Protein, cxl10 Protein, Cxcl10 Protein
- 背景
- Interferon-γ inducible protein-10 (IP-10) is a 10 kD protein that belongs to CXC chemokine family. Unlike other CXC chemokines, IP-10 is a chemoattractant for activated lymphocytes, but not resting lymphocytes or neutrophils. The gene for human IP-10 has been mapped to chromosome 4q2. CXC chemokine receptor 3 (CXCR3) has been identified as the receptor for IP-10.
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