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- 抗原 See all IL-13 (IL13) 蛋白
- IL-13 (IL13) (Interleukin 13 (IL13))
- 蛋白类型
- Recombinant
- 生物活性
- Active
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宿主
- 小鼠
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资源
- 大肠杆菌(E. Coli)
- 应用范围
- Functional Studies (Func)
- 品牌
- BD Pharmingen™
- 特异性
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Activity Assay: Measured by proliferation using TF-1 indicator cells
Specific Activity Range: 0.6 - 5 x 10^7 Units /mg
ED50: 0.2 - 1.5 ng/ml
Observed dose response relationship: 50 fold
Unit is defined as the amount of material required to stimulate a half-maximal response at cytokine saturation. - 产品特性
- Recombinant mouse IL-13 is supplied as a frozen aqueous buffered solution containing 1.0 mg/ml biotechnology grade, low endotoxin bovine serum albumin, with no preservatives. Recombinant mouse IL-13 is ≥95% pure, as determined by SDS-PAGE, and an absorbance assay based on the Beers- Lambert law.
- 纯化方法
- Recombinant mouse IL-13 is ≥95 % pure, as determined by SDS-PAGE, and an absorbance assay based on the Beers- Lambert law.
- 纯度
- ≥ 95 %
- 过滤
- 0.22 μm filtered
- 内毒素水平
- The endotoxin level is ≤ 0.1 ng per μg of mouse IL-13, as measured in a chromogenic LAL assay.
- Top Product
- Discover our top product IL13 蛋白
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 说明
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BD Pharmingen™ Recombinant Mouse IL-13
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 100 μg/mL
- 缓冲液
- Recombinant mouse IL-13 is supplied as a frozen aqueous buffered solution containing 1.0 mg/mL biotechnology grade, low endotoxin bovine serum albumin, with no preservatives.
- 储存液
- Azide free
- 注意事项
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Rapidly thaw and quick-spin product prior to use. Upon initial thawing the product should be aliquoted into polypropylene microtubes and frozen at -80°C for future use. Alternatively, prepare stock solutions diluted in sterile neutral buffer containing not less than 0.5 - 10 mg/ml carrier protein such as human or bovine serum albumin, aliquoted and stored at -80°C. For in vitro biological assay use, we recommend carrier-protein concentrations of 0.5 - 1 mg/ml. For use as an ELISA standard we recommend carrier-protein concentrations of 5 - 10 mg/ml. Failure to add carrier protein or store at indicated temperatures may result in a loss of activity. The product should not be diluted to less than 5 µg/ml for long term storage.
Note: Carrier proteins should be pre-screened for possible effects in an appropriate experimental system. Carrier proteins may effect experimental results due to toxicity, high endotoxin levels or possible blocking activity. - 储存条件
- -80 °C
- 储存方法
- Store product at -80°C prior to use or for long term storage of stock solutions. Avoid multiple freeze-thaws of product. Rapidly thaw and quick-spin product prior to use. Upon initial thawing the product should be aliquoted into polypropylene microtubes and frozen at -80°C for future use. Alternatively, prepare stock solutions diluted in sterile neutral buffer containing not less than 0.5 - 10 mg/ml carrier protein such as human or bovine serum albumin, aliquoted and stored at -80°C. For in vitro biological assay use, we recommend carrier-protein concentrations of 0.5 - 1 mg/ml. For use as an ELISA standard we recommend carrier-protein concentrations of 5 - 10 mg/ml. Failure to add carrier protein or store at indicated temperatures may result in a loss of activity. The product should not be diluted to less than 5 μg/ml for long term storage. Note: Carrier proteins should be pre-screened for possible effects in an appropriate experimental system. Carrier proteins may effect experimental results due to toxicity, high endotoxin levels or possible blocking activity.
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Modulation of murine macrophage function by IL-13." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 151, Issue 12, pp. 7151-60, (1994) (PubMed).
: "Interleukin 13, an interleukin 4-like cytokine that acts on monocytes and B cells, but not on T cells." in: Immunology today, Vol. 15, Issue 1, pp. 19-26, (1994) (PubMed).
: "Establishment and characterization of a unique human cell line that proliferates dependently on GM-CSF, IL-3, or erythropoietin." in: Journal of cellular physiology, Vol. 140, Issue 2, pp. 323-34, (1989) (PubMed).
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Modulation of murine macrophage function by IL-13." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 151, Issue 12, pp. 7151-60, (1994) (PubMed).
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- 抗原
- IL-13 (IL13) (Interleukin 13 (IL13))
- 别名
- IL-13 (IL13 产品)
- 别名
- IL13 Protein, IL-13 Protein, P600 Protein, Il-13 Protein, L13 Protein, interleukin 13 Protein, IL13 Protein, Il13 Protein
- 背景
- Interleukin-13 (IL-13) is a cytokine produced by activated T lymphocytes which regulates the function of mouse macrophages and shares some biological functions with IL-4. Administration of mouse IL-13 to cultured mouse macrophages induces morphological changes (flattening, and development of processes), leads to increases in class II MHC antigen expression, and inhibits the synthesis of several proinflammatory cytokines (including IL-1β, IL-6, TNF, and IL-12). In contrast to human IL-13, mouse IL-13 does not appear to have activity for mouse B cells. Recombinant mouse IL-13 (mIL-13) has multiple glycosylated forms ranging in molecular weights from 11.3 to 15 kD, as detected by SDS-PAGE analysis.
- 途径
- JAK/STAT Signaling, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Proton Transport
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