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- 抗原 See all C3a 蛋白
- C3a (Complement Fragment 3a (C3a))
- 蛋白类型
- Native
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宿主
- 小鼠
- 宿主
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- 应用范围
- ELISA
- 品牌
- BD Pharmingen™
- Top Product
- Discover our top product C3a 蛋白
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Complement Fragment 3a (C3a) protein
C3a 宿主: 大鼠 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % SDS, WB, ELISA, IP
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- 应用备注
- The Mouse C3a protein is useful as a standard in a sandwich ELISA to measure mouse C3a protein levels. Purified anti-mouse C3a clone I87-1162 as capture antibody can be paired with the biotinylated I87-419 clone as the detection antibody. The purified antibody should be titrated between 1-4 µg/ml to determine the optimal ELISA plate-coating concentration. For plate coating, we recommend to use a pH 6.5 phosphate buffer. To obtain linear standard curves, doubling dilutions of purified mouse C3a protein ranging from 781 pg/ml to 50 ng/ml are recommended for inclusion in each ELISA plate. For specific methodology, see Chapter 7: ELISA for specifically measuring the levels of cytokines, chemokines, and inflammatory mediators and their receptors. 2003. Techniques for Immune Function Analysis Application Handbook 1st Edition. BD Biosciences. This ELISA antibody pair shows no cross-reactivity with the following: recombinant mouse C5a, native human C3a, C4a and C5a, and rat C5a. This ELISA antibody pair shows a limited cross-reactivity with rat C3a.
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 缓冲液
- Lyophilized in an aqueous buffered solution containing BSA and ProClin™ 150.
- 储存液
- ProClin
- 注意事项
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Store the lyophilized vial at 4°C prior to reconstitution. The Mouse C3a protein was isolated from activated mouse serum by affinity chromatography. This product can be reconstituted using 1 ml of Assay Diluent (Cat. No. 555213) or equivalent neutral buffer containing carrier protein, aliquoted into single-use aliquots and stored at -80°C. Avoid multiple freeze-thaws of the reconstituted protein. Each vial contains enough protein for ten 96-well plates. Failure to add carrier protein or store at indicated temperatures may result in a loss of activity. This product should not be diluted prior to long-term storage.
- 储存条件
- 4 °C
- 储存方法
- Store the lyophilized standard at 4°C prior to reconstitution.
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- 抗原
- C3a (Complement Fragment 3a (C3a))
- 别名
- C3a (C3a 产品)
- 别名
- AHUS5 Protein, ARMD9 Protein, ASP Protein, C3a Protein, C3b Protein, CPAMD1 Protein, AI255234 Protein, HSE-MSF Protein, Plp Protein, C3d Protein, complement C3 Protein, complement component 3 Protein, C3 Protein
- 背景
- The purified Mouse C3a protein is a native protein isolated from activated mouse serum. Anaphylatoxin C3a is a bioactive cleavage product released from plasma component C3 during complement activation and is involved in mediation of a variety of cellular immune responses, as well as being a potent pro-inflammatory agent. The release of this cleavage product is a reliable indicator of in vivo or in vitro complement activation.
- 途径
- Complement System
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