MDH protein (His tag)
宿主: E. coli
宿主: 大肠杆菌（E. Coli）
Recombinant
> 95 % by SDS - PAGE
SDS
1 image
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- 抗原
- MDH
- 蛋白类型
- Recombinant
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宿主
- E. coli
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资源
- 大肠杆菌（E. Coli）
- 标记
- His tag
- 应用范围
- SDS-PAGE (SDS)
- 纯化方法
- purified by chromatography
- 纯度
- > 95 % by SDS - PAGE
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MDH (AA 1-312), (full length) protein (His tag)
宿主: E. coli 宿主: 大肠杆菌（E. Coli） Recombinant > 90 % SDS
MDH (AA 2-312) protein (His tag)宿主: Bacillus subtilis 宿主: 大肠杆菌（E. Coli） Recombinant > 90 % SDS
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- 说明
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Synonyms: malate dehydrogenase, ECK3225, JW3205
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1 mg/ml (determined by Bradford assay)
- 缓冲液
- 20 mM Tris-HCl buffer (pH 8.0) containing 1 MM DTT, 10% glycerol, 50 mM NaCl
- 储存条件
- 4 °C
- 储存方法
- Avoid repeated freezing and thawing cycles.
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- 抗原
- MDH
- 别名
- MDH Protein, MMDH Protein, malate dehydrogenase 2 Protein, MDH2 Protein
- 背景
- Malate dehydrogenase (mdh) belongs to the LDH/MDH superfamily and MDH type 1 family. This enzyme catalyzes the conversion of malate into oxaloacetate (using NAD+) and vice versa (this is a reversible reaction). Malate dehydrogenase is also involved in gluconeogenesis, the synthesis of glucose from smaller molecules. Recombinant E.coli mdh protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
- 分子量
- 34.9kDa (336aa), confirmed by MALDI-TOF
- NCBI登录号
- NP_417703
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