Recombinant Human beta-Galactoside alpha-2,6-Sialyltransferase 1/ST6GAL1 produced by transfected human cells is a secreted protein with sequence (Lys27-Cys406) of Human ST6GAL1 fused with a polyhistidine tag at the C-terminus.
纯度
> 95 % as determined by reducing SDS-PAGE.
过滤
0.2 μm filtered
内毒素水平
Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
ST6GAL1
宿主: 人
宿主: HEK-293 Cells
Recombinant
> 95 % as determined by SDS-PAGE and Coomassie blue staining
AbP, STD
限制
仅限研究用
状态
Liquid
溶解方式
It is not recommended to reconstitute to a concentration less than 100 μg/mL. Dissolve the lyophilized protein in ddH2O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
缓冲液
Supplied as a 0.2 μm filtered solution of 20 mM TrisHCl, 150 mM NaCl, pH 8.0.
注意事项
Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
储存条件
-80 °C
储存方法
Store at < -20°C, stable for 6 months after receipt. Please minimize freeze-thaw cycles.
The ST6GAL1 gene encodes beta-Galactosamide alpha-2,6-Sialyltransferase 1. It is a type II membrane protein and is localized to the trans-Golgi network. It catalyzes 2,6-sialylation of Galbeta1,4-GlcNAc structures on N-glycans. ST6GAL1 is highly expressed in the liver and other tissues. ST6GAL1 deficiency causes abnormalities in B-cell immunoreactivity. The expression and activity of ST6GAL1 are associated with tumor metastasis in breast and colon cancers. The majority of ST6GAL1 in the liver is cleaved and secreted into the serum and may be used as a biomarker for hepatitis diseases. Alternative Names: Beta-Galactoside Alpha-2,6-Sialyltransferase 1, Alpha 2,6-ST 1, B-Cell Antigen CD75, CMP-N-Acetylneuraminate-Beta-Galactosamide-Alpha-2,6-Sialyltransferase 1, ST6Gal I, ST6GalI, Sialyltransferase 1, ST6GAL1, SIAT1