Recombinant Human Serpin A12 is produced by our E. coli expression system. The target protein is expressed with sequence (Leu21-Lys414) of Human Serpin A12 fused with a GST tag at the N-terminus.
纯度
> 95 % as determined by reducing SDS-PAGE.
过滤
0.2 μm filtered
内毒素水平
Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
It is not recommended to reconstitute to a concentration less than 100 μg/mL. Dissolve the lyophilized protein in ddH2O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
缓冲液
Supplied as a 0.2 μm filtered solution of 50 mM TrisHCl, 160 mM NaCl, 0.2 mM PMSF, 1 mM DTT, 10 % Glycerine, pH 7.2 .
储存液
Dithiothreitol (DTT)
注意事项
This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项
Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
储存条件
-80 °C
储存方法
Store at < -20°C, stable for 6 months after receipt. Please minimize freeze-thaw cycles.
有效期
6 months
抗原
SERPINA12
(serpin Peptidase Inhibitor, Clade A (Alpha-1 Antiproteinase, Antitrypsin), Member 12 (SERPINA12))
SERPINA12 Protein, 4632419J12Rik Protein, OL-64 Protein, Ol-64 Protein, Ol64 Protein, serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 12 Protein, serpin family A member 12 Protein, serine (or cysteine) peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 12 Protein, serpin A12 Protein, SERPINA12 Protein, Serpina12 Protein, LOC612386 Protein
Sub Type
Fusionprotein
背景
Vaspin (Visceral Adipose-Specific SERPIN) is a newly described adipokine. Vaspin has three beta-sheets, nine alpha-helices, and one central loop, the structure is part of the set of distinctive features that are descriptive of Serpin family members. Vaspin is also a unique insulin sensitizing adipocytokine in obesity. A recent publication indicates that Vaspin mRNA expression in visceral fat is positively correlated with BMI and percent of body fat. and could be associated with parameters of obesity, insulin resistance, and glucose metabolism. These findings suggest a potential clinical use for Vaspin in ameliorating certain aberrations seen in the obesity metabolic syndrome. Alternative Names: Serpin A12, OL-64, Visceral Adipose Tissue-Derived Serine Protease Inhibitor, Vaspin, Visceral Adipose-Specific Serpin, SERPINA12