Recombinant Human Platelet-Derived Growth Factor Receptor beta/PDGFR-beta produced by transfected human cells is a secreted protein with sequence (Leu33-Phe530) of human PDGFRB fused with a polyhistidine tag at the C-terminus.
纯度
> 95 % as determined by reducing SDS-PAGE.
过滤
0.2 μm filtered
内毒素水平
Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
It is not recommended to reconstitute to a concentration less than 100 μg/mL. Dissolve the lyophilized protein in ddH2O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
缓冲液
Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.2.
注意事项
Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
储存条件
4 °C/-20 °C/-80 °C
储存方法
Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
Platelet-Derived Growth Factor Receptor beta (PDGFR-beta) is a member of the protein kinase superfamily and CSF-1/PDGF receptor subfamily. The PDGF family consists of PDGF-A, -B, -C and -D, which form either homo- or heterodimers (PDGF-AA, -AB, -BB, -CC, -DD). The four PDGFs are inactive in their monomeric forms. The PDGFs bind to the protein tyrosine kinase receptors PDGF receptor-alpha and -beta. These two receptor isoforms dimerize upon binding the PDGF dimer, leading to three possible receptor combinations, namely -alphaalpha, -betabeta and -alphabeta. The extracellular region of the PDGF receptor-beta consists of five immunoglobulin-like domains while the intracellular part is a tyrosine kinase domain. In addition to being a potent mitogen for cells of mesenchymal origin, PDGF has also been shown to be a potent chemoattractant for mesenchymal cells, mononuclear cells, and neutrophils and has been reported to be important in the modification of cellular matrix constituents. Alternative Names: Platelet-Derived Growth Factor Receptor Beta, PDGF-R-Beta, PDGFR-Beta, Beta Platelet-Derived Growth Factor Receptor, Beta-Type Platelet-Derived Growth Factor Receptor, CD140 Antigen-Like Family Member B, Platelet-Derived Growth Factor Receptor 1, PDGFR-1,