IL-2 Protein (AA 22-153)
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- 抗原 See all IL-2 (IL2) 蛋白
- IL-2 (IL2) (Interleukin 2 (IL2))
- 蛋白类型
- Recombinant
- 生物活性
- Active
- 产品特性
- AA 22-153
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宿主
- 人
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资源
- 大肠杆菌(E. Coli)
- 应用范围
- Functional Studies (Func)
- 原理
- Recombinant Human Interleukin-2/IL-2
- 序列
- MPTSSSTKKT QLQLEHLLLD LQMILNGINN YKNPKLTRML TFKFYMPKKA TELKHLQCLE EELKPLEEVL NLAQSKNFHL RPRDLISNIN VIVLELKGSE TTFMCEYADE TATIVEFLNR WITFCQSIIS TLT
- 产品特性
- Recombinant Human Interleukin-2/IL-2 is produced by our E. coli expression system. The target protein is expressed with sequence (Pro22-Thr153) of Human IL-2.
- 纯度
- > 95 % as determined by reducing SDS-PAGE.
- 过滤
- 0.2 μm filtered
- 内毒素水平
- Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
- Top Product
- Discover our top product IL2 蛋白
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- 说明
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Biological activity: ED50 is less than 0.1 ng/ml as determined by the dose-dependent stimulation of murine CTLL-2 cells.Specific Activity of 1.0 x 107 IU/ mg.
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
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It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles. - 缓冲液
- Lyophilized from a 0.2 μm filtered solution of 20 mM HAc-NaAc, 150 mM NaCl, pH 4.0.
- 注意事项
- Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
- 储存条件
- 4 °C/-20 °C/-80 °C
- 储存方法
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Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months. - 有效期
- 3 months
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- 抗原
- IL-2 (IL2) (Interleukin 2 (IL2))
- 别名
- interleukin-2 (IL2 产品)
- 别名
- IL2 Protein, IL-2 Protein, il2 Protein, TCGF Protein, lymphokine Protein, Il-2 Protein, POIL2 Protein, interleukin 2 Protein, interleukin-15 Protein, IL2 Protein, il2 Protein, LOC100303688 Protein, Il2 Protein
- 背景
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Recombinant Human Interleukin-2 is a highly purified protein with a molecular weight of approximately 15,300 Daltons. The chemical name is des-alanyl-1, serine-125 Human Interleukin-2. It is produced by recombinant DNA technology using a genetically engineered E. coli strain containing an analog of the human interleukin-2 gene. Genetic engineering techniques were used to modify the Human IL-2 gene, and the resulting expression clone encodes a modified Human IL-2. This recombinant form differs from native Interleukin-2 in following ways: 1) it is not glycosylated, 2) the molecule has no N-terminal alanine, 3) the molecule has serine substituted for cysteine at amino acid position 125, 4) the aggregation state of molecule is likely to be different from that of native IL-2.
Alternative Names: Interleukin-2, IL-2, T-Cell Growth Factor, TCGF, Aldesleukin, IL2 - 分子量
- 15.4 kDa
- UniProt
- P60568
- 途径
- JAK/STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Activated T Cell Proliferation
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