Recombinant Human Chymotrypsin-C/CTRC produced by transfected human cells is a secreted protein with sequence (Cys17-Leu268) of Human CTRC fused with a polyhistidine tag at the C-terminus.
纯度
> 95 % as determined by reducing SDS-PAGE.
过滤
0.2 μm filtered
内毒素水平
Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
CTRC
宿主: 人
宿主: HEK-293 Cells
Recombinant
> 95 % as determined by SDS-PAGE and Coomassie blue staining
AbP, STD
限制
仅限研究用
状态
Liquid
溶解方式
It is not recommended to reconstitute to a concentration less than 100 μg/mL. Dissolve the lyophilized protein in ddH2O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
缓冲液
Supplied as a 0.2 μm filtered solution of 20 mM TrisHCl, 150 mM NaCl, pH 8.0.
注意事项
Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
储存条件
-80 °C
储存方法
Store at < -20°C, stable for 6 months after receipt. Please minimize freeze-thaw cycles.
有效期
6 months
抗原
Elastase 4 (CTRC)
(Chymotrypsin C (Caldecrin) (CTRC))
CLCR Protein, ELA4 Protein, 1810044E12Rik Protein, bPTLP Protein, chymotrypsin C Protein, chymotrypsin-C Protein, chymotrypsin C (caldecrin) Protein, CTRC Protein, LOC478220 Protein, Ctrc Protein
Sub Type
Fusionprotein
背景
Chymotrypsin C (CTRC) is a member of the peptidase S1 family. CTRC is a serum calcium-decreasing factor that has chymotrypsin-like protease activity. CTRC has broad substrate specificity, but prefers to cleave on the carboxyl side of hydrophobic residues. CTRC is expressed primarily in the pancreas, and is secreted into the digestive tract. CTRC plays a protective role in the pancreas by mitigating premature trypsinogen activation through degradation. It has been proposed that CTRC is a key regulator of digestive zymogen activation and is a physiological coactivator of digestive carboxypeptidases proCPA1 and proCPA2. The mutation of CTRC gene encodes the digestive enzyme CTRC contribute to the development of pancreatitis. Alternative Names: Chymotrypsin-C, Caldecrin, CTRC, CLCR