Recombinant Human beta-Defensin 4/DEFB4 is produced with our E. coli expression system. The target protein is expressed with sequence (Glu23-Pro72) of Human beta-Defensin 4.
纯度
> 95 % as determined by reducing SDS-PAGE.
过滤
0.2 μm filtered
内毒素水平
Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
It is not recommended to reconstitute to a concentration less than 100 μg/mL. Dissolve the lyophilized protein in ddH2O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
缓冲液
Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 130 mM NaCl, pH 7.4.
注意事项
Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
储存条件
4 °C/-20 °C/-80 °C
储存方法
Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
Defensins are cationic peptides. It is an important ingredient of the innate immune system. beta-defensins are expressed on some leukocytes and epithelial surfaces. Four human beta-Defensins have been identified to date: BD-1, BD-2, BD-3 and BD-4. beta-defensins contain a six-cysteine motif, they forms three intra-molecular disulfide bonds. beta-defensins are also chemoattractant towards immature dendritic cells and memory T cells. The beta-defensin proteins are expressed as the C-terminal portion of precursors, they are released by proteolytic cleavage of a signal sequence. Alternative Names: Beta-Defensin 104, Beta-Defensin 4, BD-4, DEFB-4, hBD-4, Defensin Beta 104, DEFB104A, DEFB104, DEFB4, DEFB104B