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- 抗原 See all Annexin V (ANXA5) 蛋白
- Annexin V (ANXA5) (Annexin A5 (ANXA5))
- 蛋白类型
- Recombinant
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宿主
- 小鼠
- 宿主
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- 标记
- This Annexin V protein is labelled with Alexa Fluor 488.
- 产品特性
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Synonyms: Annexin-5, Lipocortin V, Endonexin II, Calphobindin I (CBP-I), Placental anticoagulant protein I (PAP-I), Placental anticoagulant protein 4 (PP4), Thromboplastin inhibitor, Vascular anticoagulant-alpha (VAC-alpha), Anchorin CII Conjugate: Alexa Fluor 488 Maximum of Absorption at 495 nm, Maximum of Emission at 519 nm
Gene Name: Anxa5 - 试剂未包括
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Binding Buffer: 10 mM Hepes pH7.4, 140 mM NaCl, 2.5 mM CaCl2
Propidium iodide: 50 g/ml, final concentration 1 g/ml - Top Product
- Discover our top product ANXA5 蛋白
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Annexin A5 (ANXA5) (AA 2-320) protein
ANXA5 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Annexin A5 (ANXA5) (AA 1-319) protein (His-EGFP Tag)ANXA5 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Annexin A5 (ANXA5) (AA 1-320) protein (GST tag)ANXA5 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Annexin A5 (ANXA5) protein (FITC)ANXA5 宿主: Bacteria > 99 % (SDS-PAGE)
Annexin A5 (ANXA5) (AA 1-320) proteinANXA5 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % pure SDS
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- 应用备注
- 5 l are suitable for one assay
- 实验流程
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Staining protocol:
1. Harvest 2x105 cells per staining and wash cells twice in cold phosphate- buffered-saline (PBS)
2. Resuspend cells in 100 l binding buffer, add 5l annexin V-Alexa488 to the cells and mix gently.
For detection of necrosis add additionally appropriate amounts of propidium iodide (final concentration 1g/ml or 1.5M).
3. Incubate for 15min at room temperature in the dark
4. Add 400 l binding buffer and analyse the staining in flow cytometry as soon as possible (within an hour). - 限制
- 仅限研究用
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- 状态
- Liquid
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
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: "A novel assay for apoptosis. Flow cytometric detection of phosphatidylserine expression on early apoptotic cells using fluorescein labelled Annexin V." in: Journal of immunological methods, Vol. 184, Issue 1, pp. 39-51, (1995) (PubMed).
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: "A novel assay for apoptosis. Flow cytometric detection of phosphatidylserine expression on early apoptotic cells using fluorescein labelled Annexin V." in: Journal of immunological methods, Vol. 184, Issue 1, pp. 39-51, (1995) (PubMed).
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- 抗原
- Annexin V (ANXA5) (Annexin A5 (ANXA5))
- 别名
- Annexin V-Alexa488 (ANXA5 产品)
- 别名
- anx Protein, anx5 Protein, ANX V Protein, anxa5 Protein, cb989 Protein, wu:fa98f06 Protein, wu:fj10f10 Protein, MGC89158 Protein, ANX5 Protein, ENX2 Protein, PP4 Protein, RPRGL3 Protein, Anx5 Protein, R74653 Protein, LC5 Protein, enx2 Protein, annexin A5 Protein, annexin A5b Protein, Annexin A5 Protein, annexin A5 L homeolog Protein, ANXA5 Protein, anxa5b Protein, anxa5 Protein, Anxa5 Protein, anxa5.L Protein
- 基因ID
- 11747
- UniProt
- P48036
- 途径
- Apoptosis
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