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Protein A Protein (HRP)

宿主: Staphylococcus aureus ELISA, WB
产品编号 ABIN1044879
发货至: 中国
  • 抗原 See all Protein A 蛋白
    Protein A
    宿主
    Staphylococcus aureus
    宿主
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    标记
    This Protein A protein is labelled with HRP.
    应用范围
    ELISA, Western Blotting (WB)
    特异性
    Protein A Peroxidase was prepared from chromatographically pure Protein A. Protein A Peroxidase was assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase and anti-Protein A. No reaction was observed against anti-Protein G.
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    Discover our top product Protein A 蛋白
  • 应用备注
    Protein A Peroxidase is a useful reagent in Western Blotting and ELISA experiments. Protein A Peroxidase can be utilized as a pseudo-secondary detection reagent when used in conjunction with an IgG-based primary antibody and appropriate substrate (such as TMB-1000 or Femtomax-110). Protein A Peroxidase is stable at 4° C until restored, at which point it is recommended to freeze aliquots at -20° C.
    说明

    Protein A Peroxidase is a useful reagent in Western Blotting and ELISA experiments. Protein A Peroxidase can be utilized as a pseudo-secondary detection reagent when used in conjunction with an IgG-based primary antibody and appropriate substrate (such as TMB-1000 or Femtomax-110). Protein A Peroxidase is stable at 4° C until restored, at which point it is recommended to freeze aliquots at -20° C.

    限制
    仅限研究用
  • 状态
    Lyophilized
    溶解方式
    Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 1.0 mL
    浓度
    1.0 mg/mL
    缓冲液
    0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
    储存条件
    4 °C
    有效期
    12 months
  • 抗原
    Protein A
    Abstract
    Protein A 产品
    别名
    alpha protein Protein, repB Protein
    背景
    Protein A is a surface protein (approximately 56kDa in size) originally discovered within the cell wall of Staphylococcus aureus. While important for bacterial survival, Protein A has beneficial uses in immunology for its high affinity binding to immunoglobulins (especially the IgG isotype). This high affinity property makes Protein A essential in the large scale purification of antibodies. Horseradish Peroxidase (HRP) is an enzyme that utilize organic peroxide compounds as electron donors. Naturally provides protection for plants against pathogens, but can be utilized in molecular biology to convert various substrates to detectable compounds (such as in Western Blotting and ELISAs).
    Synonyms: HRP, peroxidase, Horseradish peroxidase protein A
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