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PrxQ 抗体

PRXQ 适用: Arabidopsis thaliana, Manihot esculenta, Spinach, Zea mays WB 宿主: 兔 Polyclonal unconjugated
产品编号 ABIN125852
发货至: 中国
  • 抗原
    PrxQ (PRXQ) (Peroxiredoxin Q, Thioredoxin Dependent Peroxidase Chloroplast (PRXQ))
    适用
    Arabidopsis thaliana, Manihot esculenta, Spinach, Zea mays
    宿主
    克隆类型
    多克隆
    应用范围
    Western Blotting (WB)
    交叉反应 (详细)
    Not reactive in: no confirmed exceptions from predicted reactivity known in the moment
    预测反应
    Populus spp. , monocots including Triticum aestivum, Oryza sativa
    产品特性
    Expected / apparent Molecular Weight of the Antigene: 16 kDA
    纯化方法
    serum
    免疫原
    His-tagged full length protein (with presequence) of Arabidopsis thaliana was overexpressed in in E.coli. Isolated with HiTrap column (GE Healthcare) Q9LU86, At3g26060
  • 应用备注
    1: 5000 with standard ECL (WB)
    说明

    In stroma fractions a weak background reaction at 28 kDa is visible. No crossreactivity in any thylakoid fractions.

    限制
    仅限研究用
  • 状态
    Lyophilized
    溶解方式
    For reconstitution add 200 μL of sterile water.
    注意事项
    Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
    Once reconstituted make aliquots to avoid repreated freeze-thaw cycles.
    储存条件
    -20 °C
    储存方法
    store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
  • Wu, Han, Wang, Wang, Sun, Zu, Chen: "Comparative proteomic analysis of the plant-virus interaction in resistant and susceptible ecotypes of maize infected with sugarcane mosaic virus." in: Journal of proteomics, Vol. 89, pp. 124-40, (2013) (PubMed).

    Pulido, Spuinola, Kirchsteiger, Guinea, Pascual, Sahrawy, Sandalio, Dietz, Gonzualez, Cejudo: "Functional analysis of the pathways for 2-Cys peroxiredoxin reduction in Arabidopsis thaliana chloroplasts." in: Journal of experimental botany, (2010) (PubMed).

    Lehtima: "Nodularin uptake and induction of oxidative stress in spinach (Spinachia oleracea)." in: Journal of plant physiology, (2010) (PubMed).

  • 抗原
    PrxQ (PRXQ) (Peroxiredoxin Q, Thioredoxin Dependent Peroxidase Chloroplast (PRXQ))
    别名
    PrxQ
    背景
    AGI Code: At3g26060
    Peroxiredoxins (EC=1.11.1.15) belong to the enzyme family which is ubiquitous in all kingdoms of life. Prx Q enzyme acting by reducing hydroperoxides. Peroxiredoxins have no heme group, unlike the other peroxidases, but perform their enzymatic activity using cysteine residues with redox-active thiol groups. The ability of peroxiredoxins to hydrolyze hydroperoxides suggests that this protein family has a general function in oxidant defence.
    分子量
    16 kDA
    UniProt
    Q9LU86
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