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PRDX6-PS2 抗体 (C-Term)

PRDX6-PS2 适用: Arabidopsis thaliana ELISA, WB 宿主: 兔 Polyclonal unconjugated
产品编号 ABIN125822
发货至: 中国
  • 抗原
    PRDX6-PS2 (Peroxiredoxin 6 Pseudogene 2 (PRDX6-PS2))
    抗原表位
    C-Term
    适用
    Arabidopsis thaliana
    宿主
    克隆类型
    多克隆
    应用范围
    ELISA, Western Blotting (WB)
    交叉反应 (详细)
    Not reactive in: no confirmed exceptions from predicted reactivity known in the moment
    产品特性
    Expected / apparent Molecular Weight of the Antigene: 25 / 20 kDa
    纯化方法
    serum
    免疫原
    thioredoxin fusion of 58 amino acid region between Ser at position 175 and the C-terminal Ala of AtGPX7 accession At4g31870 has been used for antibody production
  • 应用备注
    1: 5000 with alkaline phosphatase system (AP) (WB), 1:1, 1:2, 1:4, 1:8, 1: 16 (ELISA)
    说明

    This antibody is recognizing Arabidopsis thaliana GPX (two chloroplastic isoforms). Antibody has also a weak cross-reactivity to cytosolic forms, therefore work on isolated chloroplasts is recommended.

    限制
    仅限研究用
  • 状态
    Lyophilized
    溶解方式
    For reconstitution add 100 μL of sterile water.
    注意事项
    Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
    Once reconstituted make aliquots to avoid repreated freeze-thaw cycles.
    储存条件
    -20 °C
    储存方法
    store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
  • Lepistö, Pakula, Toivola, Krieger-Liszkay, Vignols, Rintamäki: "Deletion of chloroplast NADPH-dependent thioredoxin reductase results in inability to regulate starch synthesis and causes stunted growth under short-day photoperiods." in: Journal of experimental botany, Vol. 64, Issue 12, pp. 3843-54, (2013) (PubMed).

    Kim, Jin, Yoon: "Decarbonylated cyclophilin A Cpr1 protein protects Saccharomyces cerevisiae KNU5377Y when exposed to stress induced by menadione." in: Cell stress & chaperones, (2010) (PubMed).

  • 抗原
    PRDX6-PS2 (Peroxiredoxin 6 Pseudogene 2 (PRDX6-PS2))
    别名
    GPX
    背景
    AGI Code: At4g31870
    GPX belongs to the group of enzymes that protect cells against oxidative damage generated by reactive oxygen species (ROS). Glutathione peroxidase catalyzes the reduction of H2O2, organic hydroperoxides, and lipid peroxides in the presence of glutathione, the hydrogen donor. Activity of the enzyme can be increased under environmental stress. Immunogen: Peptide target chosen from C-terminal of two chloroplastic isoforms of Arabidopsis thaliana GPX.
    分子量
    expected: 25 kDa, apparent: 20 kDa
    UniProt
    Q9SZ54
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