Purified recombinant Human Thioredoxin 2 protein Expression System: E.coli Bioactivity: Specific activity is 3-4 A650/min/mg, obtained by measuring the increase of insulin precipitation in absorbance at 650 nm resulting from the reduction of insulin. Molecular weight on SDS-PAGE will appear higher.
Thioredoxin 2 protein has been used in SDS PAGE and may be suitable for use in other assays to be determined by the end user.
实验流程
Prepare a 1 mL reaction cocktail in a suitable container: The final concentrations are 1 mM Tris, 61 mM sodium phosphate, 1 mM DTT, 0.1 % (w/v) insulin, 1 mM EDTA. 2. Add 4 µg of recombinant thioredoxin-2 into reaction cocktail and mix immediately. 3. Incubate at 25 °C for 20 minutes. 4. Record the increase in A650 nm for 15 minutes.
限制
仅限研究用
状态
Liquid
浓度
1 mg/mL
缓冲液
Supplied as a liquid in PBS, pH 7.4.
注意事项
Avoid repeated freeze/thaw cycles.
储存条件
RT/-20 °C
储存方法
Store at 4 °C for short term storage (1/2 weeks). Aliquot and store at -20 °C or - 70 °C for long term storage.
Thioredoxin(Trx) is a low molecular weight redox protein. Trx contains a redox active disulfide/dithiol group within the conserved Cys-Gly-Pro-Cys active site. The Thioredoxin 2 may play important roles in the regulation of the mitochondrial membrane potential and in protection against oxidant-induced apoptosis. Recombinant Thioredoxin 2 was expressed in E. coli and purified by using conventional chromatography techniques. Alternative Names: Thioredoxin 2, MTRX protein, MT-TRX protein, Thioredoxin2 protein, TXN 2 protein, Thioredoxin mitochondrial protein, Thioredoxin-2, Thioredoxin 2 protein, thioredoxin 2 precursor protein, mitochondrial thioredoxin protein, Thioredoxin -2, TRX2 protein, TRX 2 protein, Thioredoxin -2 protein, Thioredoxin 2, TXN2 protein