When peptides are reconstituted in 200 μL water, the concentration would be also 0.5 mg/mL. To start, the best ratio would be 1:1 (which means molar excess of peptides relative to antibodies when identical volumes are mixed). Please mix equal volumes of peptide and antibody at the required dilution and leave at ambient temperature. Best is to have two identical blots, to be incubated with equal amount of antibodies, but one with the antibodies pre-adsorbed to the peptide for 20 min. Then incubate and develop in parallel the two blots.Approx 26 kDa band observed in Human Adipose lysates (calculated MW of 27.0 kDa according to NP_001919.2). Recommended concentration: 1-3 μg/mL.
限制
仅限研究用
状态
Lyophilized
溶解方式
Reconstitute pellet in distilled water where required. For Blocking studies, reconstitute pellet in 200µl distilled water to obtain a 0.5mg/ml peptide solution.
缓冲液
100µg of Lyophilised peptide, formulation De-ionized (DI) water.
储存条件
-20 °C
储存方法
Shipped at ambient temperature, store at -20°C.
抗原
Adipsin (CFD)
(Complement Factor D (CFD))
别名
ADIPSIN Peptide, ADN Peptide, DF Peptide, PFD Peptide, Adn Peptide, Df Peptide, EVE Peptide, CFD Peptide, adipsin Peptide, pfd Peptide, cfdl Peptide, wu:fb61f12 Peptide, zgc:109940 Peptide, 10.5 Peptide, 10.9 Peptide, 14.10 Peptide, 20.35 Peptide, CG2328 Peptide, Dmel\\CG2328 Peptide, E(eve) Peptide, Eve Peptide, F Peptide, V Peptide, VI Peptide, eve2 Peptide, even Peptide, l(2)46CFg Peptide, l(2)46CFh Peptide, l(2)46CFj Peptide, l(2)46CFp Peptide, l(2)46Ce Peptide, l(2)46Cg Peptide, complement factor D Peptide, complement factor D (adipsin) Peptide, complement factor D (adipsin) L homeolog Peptide, even skipped Peptide, CFD Peptide, Cfd Peptide, cfd.L Peptide, cfd Peptide, eve Peptide