(5 references)-
- 抗原 See all DYKDDDDK Tag products
- DYKDDDDK Tag
- 纯度
- > 95 %
-
DYKDDDDK Tag peptide (DYKDDDDK Tag)
适用: 标记 CP, IP, WB
DYKDDDDK Tag peptide适用: 标记 CP, IP, WB
-
- 限制
- 仅限研究用
-
- 储存条件
- 4 °C
-
-
: "USP7 controls Chk1 protein stability by direct deubiquitination." in: Cell cycle (Georgetown, Tex.), Vol. 13, Issue 24, pp. 3921-6, (2015) (PubMed).
: "Assembly and architecture of the EBV B cell entry triggering complex." in: PLoS pathogens, Vol. 10, Issue 8, pp. e1004309, (2014) (PubMed).
: "An essential role for chloroplast heat shock protein 90 (Hsp90C) in protein import into chloroplasts." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 110, Issue 8, pp. 3173-8, (2013) (PubMed).
: "A lysine-rich region within fungal BAG domain-containing proteins mediates a novel association with ribosomes." in: Eukaryotic cell, Vol. 11, Issue 8, pp. 1003-11, (2012) (PubMed).
: "In vivo and in vitro analysis of the hedamycin polyketide synthase." in: Chemistry & biology, Vol. 16, Issue 11, pp. 1197-207, (2009) (PubMed).
-
: "USP7 controls Chk1 protein stability by direct deubiquitination." in: Cell cycle (Georgetown, Tex.), Vol. 13, Issue 24, pp. 3921-6, (2015) (PubMed).
-
- 抗原
- DYKDDDDK Tag
- 物质类
- Tag
- 背景
- A fusion tag, called DYKDDDDK and consisting of eight amino acids (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) including an enterokinase-cleavage site, was specifically designed for immunoaffinity chromatography. It allows elution under non-denaturing conditions. Several antibodies against this peptide have been developed. One antibody, denoted as M1, binds the peptide in the presence of bivalent metal cations, preferably Ca2+. Elution is effected by chelating agents. Another strategy is competitive elution with excess of free DYKDDDDK peptide.
-
You are here:
