When peptides are reconstituted in 200ul water, the concentration would be also 0.5mg/ml. To start, the best ratio would be 1:1 (which means molar excess of peptides relative to antibodies when identical volumes are mixed). Please mix equal volumes of peptide and antibody at the required dilution and leave at ambient temperature. Best is to have two identical blots, to be incubated with equal amount of antibodies, but one with the antibodies pre-adsorbed to the peptide for 20min. Then incubate and develop in parallel the two blots.
限制
仅限研究用
状态
Lyophilized
溶解方式
Reconstitute pellet in distilled water where required. For Blocking studies, reconstitute pellet in 200µl distilled water to obtain a 0.5mg/ml peptide solution.
缓冲液
100µg of Lyophilised peptide, formulation De-ionized (DI) water.
储存条件
-20 °C
储存方法
Shipped at ambient temperature, store at -20°C.
抗原
RNF8
(Ring Finger Protein 8 (RNF8))
别名
hRNF8 Peptide, 3830404E21Rik Peptide, AIP37 Peptide, RNF8 Peptide, rnf8 Peptide, fc66f09 Peptide, wu:fc66f09 Peptide, zgc:55936 Peptide, zgc:77313 Peptide, ring finger protein 8 Peptide, ring finger protein 8, E3 ubiquitin protein ligase S homeolog Peptide, ring finger protein 8, E3 ubiquitin protein ligase L homeolog Peptide, ring finger protein 8, E3 ubiquitin protein ligase Peptide, RNF8 Peptide, Rnf8 Peptide, rnf8.S Peptide, rnf8.L Peptide, rnf8 Peptide