A working concentration of 500 μg/mL for triple DYKDDDDK peptide is typically used for competitive elution of DYKDDDDK containing proteins from anti-DYKDDDDK affinity gels (p/n 200-350-383). For use as a control in antibody-peptide competition assays (PCA) use the peptide at 1.0 µg per 1.0 µl of antibody/antiserum in per assay. See anti-DYKDDDDK DYKDDDDK affinity gel PROTOCOL for specific instructions for use.
限制
仅限研究用
状态
Lyophilized
溶解方式
0.05 M Tris Chloride, 0.15 M Sodium Chloride, pH 7.4
浓度
1 mg/mL
储存条件
4 °C/-20 °C
储存方法
Store vial at 2 - 8 °C prior to opening. Aliquot contents and freeze at -20 °C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Dilute only prior to immediate use.
有效期
Expiration date is six (6) months from date of opening.
抗原
DYKDDDDK Tag
物质类
Tag
背景
The DYKDDDDK peptide (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) is intended for immunoaffinity chromatography to allow elution under non-denaturing conditions by competitive elution with excess of free DYKDDDDK Peptide. Synonyms: synthetic DYKDDDDK peptide, ddddk peptide, dykddddk peptide, Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys, control peptide