小鼠 CXCL4/PF4 EZ Set™ ELISA Kit (DIY Antibody Pairs)
-
- 抗原 See all PF4 products
- PF4 (Platelet Factor 4 (PF4))
- 抗原表位
- AA 30-105
- 适用
- 小鼠
- 检测方法
- Colorimetric
-
宿主
- 兔
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 原理
- EZ Set™ ELISA kit containing the core components for developing solid phase sandwich ELISA assay to quantitatively detects CXCL4/PF4 in Mouse samples.
- 品牌
- EZ-Set™
- 特异性
-
Expression system for standard: E.coli
Immunogen sequence: V30-S105 - 交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
- 组件
- Capture Antibody, Biotinylated Detection Antibody, Lyophilized Standard, Avidin-Biotin-Peroxidase Complex (ABC)
- 试剂未包括
-
1. Microplate reader in standard size.
2. Automated plate washer.
3. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
4. Clean tubes and Eppendorf tubes.
5. Washing buffer (neutral PBS or TBS).
-
PF4 (Human) IP-WB Antibody Pair
IP 适用: 人 IP unconjugated 宿主: 兔, 小鼠
-
- 说明
-
The EZ Set™ ELISA antibody pair set contains the necessary core reagents to develop and run an ELISA, including ELISA-optimized matched antibody pairs, recombinant protein standards, and HRP conjugated Avidin-Biotin-Complex (ABC).
The remaining necessary components could be obtained via the EZ Set™ Accessory Kit (ABIN5510802)
The EZ Set™ Accessory Kit provides:
Antibody diluent buffer
ABC diluent buffer
Sample diluent buffer
TMB
TMB stop solution
5x 96 well plates, plate sealers - 限制
- 仅限研究用
-
- 缓冲液
-
Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2O and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
Preparation of 0.01M PBS: Add 8.5g sodium chloride, 1.4g Na2HPO4 and 0.2g NaH2PO4 to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L. - 储存条件
- 4 °C/-20 °C
- 储存方法
- Store at 4° for 6 months, at -20° for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
- 有效期
- 12 months
-
- 抗原
- PF4 (Platelet Factor 4 (PF4))
- 别名
- Pf4 (PF4 产品)
- 别名
- CXCL4 Matched Antibody Pair, PF-4 Matched Antibody Pair, SCYB4 Matched Antibody Pair, Cxcl4 Matched Antibody Pair, Scyb4 Matched Antibody Pair, Pf4a Matched Antibody Pair, RATPF4A Matched Antibody Pair, PF4A Matched Antibody Pair, CXCL4L1 Matched Antibody Pair, CXCL4V1 Matched Antibody Pair, PF4-ALT Matched Antibody Pair, SCYB4V1 Matched Antibody Pair, PF4 Matched Antibody Pair, platelet factor 4 Matched Antibody Pair, platelet factor 4 variant 1 Matched Antibody Pair, PF4 Matched Antibody Pair, Pf4 Matched Antibody Pair, LOC703451 Matched Antibody Pair, PF4V1 Matched Antibody Pair, LOC100524373 Matched Antibody Pair, LOC102181854 Matched Antibody Pair, LOC100630489 Matched Antibody Pair
- 背景
-
Protein Function: Released during platelet aggregation. Neutralizes the anticoagulant effect of heparin because it binds more strongly to heparin than to the chondroitin-4-sulfate chains of the carrier molecule. Chemotactic for neutrophils and monocytes. Inhibits endothelial cell proliferation (By similarity). .
Background: Platelet factor 4(PF4) is a small cytokine belonging to the CXC chemokine family that is also known as chemokine(C-X-C motif) ligand 4(CXCL4). The PF4 gene was localized on map to 4q12-q13. Chemokines play fundamental roles in the development, homeostasis, and function of the immune system, and they have effects on cells of the central nervous system as well as on endothelial cells involved in angiogenesis or angiostasis. Platelet factor-4 is a 70-amino acid protein that is released from the alpha-granules of activated platelets and binds with high affinity to heparin. Its major physiologic role appears to be neutralization of heparin-like molecules on the endothelial surface of blood vessels, thereby inhibiting local antithrombin III activity and promoting coagulation. As a strong chemoattractant for neutrophils and fibroblasts, PF4 probably has a role in inflammation and wound repair.
Synonyms: Platelet factor 4,PF-4,C-X-C motif chemokine 4,Pf4,Cxcl4, Scyb4,
Full Gene Name: Platelet factor 4
Cellular Localisation: Secreted. - UniProt
- Q9Z126
-
