Nuclear Receptor Subfamily 1, Group H, Member 2/3 (NR1H2/3) (AA 429-442), (AA 443-456) 抗体
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- 抗原 See all Nuclear Receptor Subfamily 1, Group H, Member 2/3 (NR1H2/3) products
- Nuclear Receptor Subfamily 1, Group H, Member 2/3 (NR1H2/3)
- 抗原表位
- AA 429-442, AA 443-456
- 适用
- 人
- 宿主
- 山羊
- 克隆类型
- 多克隆
- 应用范围
- ELISA, Flow Cytometry (FACS), Immunofluorescence (IF), Western Blotting (WB)
- 原理
- LXR alpha / LXR beta
- 序列
- CRLQDKKLPP LLSEI
- 特异性
- This antibody is expected to recognise epitopes including aa 429-442 of human LXR alpha protein (NP_005684.2) and aa 443-456 of human LXR beta protein (NP_009052.3).
- 交叉反应
- Cow, 犬, 人, 小鼠, 大鼠
- 纯化方法
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- 质量等级
- Verified
- 免疫原
- Peptide with sequence CRLQDKKLPPLLSEI, from the internal region of the protein sequence according to NP_005684.2, NP_009052.3.
- 亚型
- IgG
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- 应用备注
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Western Blot: Approx 55 kDa band observed in Human, Liver and Kidney lysates and in preliminary testing of Human Spleen lysate (calculated MW of 51.1 kDa according to NP_001238863.1). This band was successfully blocked by incubation with the immunizing pe
Peptide ELISA: antibody detection limit dilution 1:32000.
- 说明
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Immunofluorescence: Strong expression of the protein seen in the nuclei of A549 cells. Recommended concentration: 10μg/ml.
Flow Cytometry: Flow cytometric analysis of A549 cells. Recommended concentration: 10ug/ml.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 0.5 mg/mL
- 缓冲液
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Minimize freezing and thawing.
- 储存条件
- -20 °C
- 储存方法
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- 抗原
- Nuclear Receptor Subfamily 1, Group H, Member 2/3 (NR1H2/3)
- 别名
- NR1H3, NR1H2
- 别名
- LXR-a antibody, LXRA antibody, RLD-1 antibody, nuclear receptor subfamily 1 group H member 3 antibody, NR1H3 antibody
- 背景
- NR1H3, LXRA, LXR-a, RLD-1, nuclear receptor subfamily 1, group H, member 3, liver X receptor, alpha, NR1H2, NER, UNR, LXR-b, NER-I, RIP15, nuclear receptor subfamily 1, group H, member 2, ubiquitously-expressed nuclear receptor / NR1H2, NER, UNR, LXR-b, N
- 基因ID
- 10062, 7376
- NCBI登录号
- NP_005684, NP_009052, NP_001123573, NP_001238863, NP_001350524
- 途径
- Nuclear Receptor Transcription Pathway, Cellular Response to Molecule of Bacterial Origin, Hepatitis C
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