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Nlrp4e Protein (AA 1-978) (Strep Tag)

NLRP4E 宿主: 小鼠 宿主: Tobacco (Nicotiana tabacum) Recombinant > 80 % as determined by SDS PAGE, Western Blot and analytical SEC (HPLC). ELISA, SDS, WB
产品编号 ABIN3135621
发货至: 中国
  • 抗原
    Nlrp4e (NLRP4E) (NLR Family, Pyrin Domain Containing 4E (NLRP4E))
    蛋白类型
    Recombinant
    产品特性
    AA 1-978
    宿主
    小鼠
    资源
    Tobacco (Nicotiana tabacum)
    标记
    This Nlrp4e protein is labelled with Strep Tag.
    应用范围
    ELISA, SDS-PAGE (SDS), Western Blotting (WB)
    序列
    MASFFSDFGL MWYLEELNKK EFMKFKELLQ QEILQLGLKH ISWTEVKKAS REDLANLLLK HYEEKKAWDM TFKIFQKMNR QDLMERAGRE IAGHSKLYQV HLKKKLTHDY ARKFNIKDQD SLKQKFTQDD CDHFENLLIS KATGKKPHMV FLQGVAGIGK SLMLTKLMLA WSEGMVFQNK FSYIFYFCCQ DVKKMKRASL AELISKEWPN ASAPIEEILS QPEKLLFVID NLEVMECDMS EWESELCDDC MEKQPVNILM SSLLRRKMLP ESSFLVSATP ETFEKIEDRI ECTNVKMMAG FNESSIKVYF YSLFQDRNRT QEIFSLVREN EQLFSVCQVP VLCWMVATCL KKEIEKGRDP VSICRRITSL YTTYIFNLFI PHSAQYPSKK SQDQLQGLCS LAAEGMWTDT FVFAEEALRR NGIMDSDIST LLDVRILEKS KESEKSYIFL HPSIQEVCAA IFHLLKSHVD HPSQDVKSIE ALIFTFLKKV KIQWIFFGSF IFGLLHESEQ KKLEAFFGHQ LSQEIKRQLY QCLETISGNK ELQEQIDGMK LFYCLFEMDD EAFLPQVMNC MEQIKFVAKD YSDVIVAAHC LQHCSTLKKL SLSTQNILSE GQEHSYTEKL LICWHHVCSV LTSSKDIHVL QVKDTNFNER AFLVLYSHLK YPSCILKVLE VNNVTLLCDN RLLFELIQNQ RLQLLNLSLT FLSHNDVKLL CDVLNQAECN IEKLMVADCN LSPDDCKVFV SVLISSKMLK HLNLSSNNLD KGISSLSKAL CHPDCVLKNL VLAKCSLSEE CWHYLSEVLR RNKTLTHLDI SFNDLKDEGL KVLCGALTLP DSVLISLSVR YCLITTSGCQ DLAEVLRNNQ NLRNLQISNN KIEDAGVKLL CDAIKHPNCH LENIGLEACA LTGACCEDLA SSFTHCKTLL GINLQENALD HSGLVALFEA MKQQQCTVNL RGLRITDFDK ETQEFLMAEK EKNPYLSI
    Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.
    产品特性
    Key Benefits:
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Protein expressed with ALiCE® and purified in one-step affinity chromatography
    • These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.


    Expression System:
    • ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
    • During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

    Concentration:
    • The concentration of our recombinant proteins is measured using the absorbance at 280nm.
    • The protein's absorbance will be measured against its specific reference buffer.
    • We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.

    纯化方法
    One-step Strep-tag purification of proteins expressed in Almost Living Cell-Free Expression System (AliCE®).
    纯度
    > 80 % as determined by SDS PAGE, Western Blot and analytical SEC (HPLC).
  • 应用备注
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
    说明

    ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
    During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

    限制
    仅限研究用
  • 状态
    Liquid
    缓冲液
    The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.
    注意事项
    Avoid repeated freeze-thaw cycles.
    储存条件
    -80 °C
    储存方法
    Store at -80°C.
    有效期
    Unlimited (if stored properly)
  • 抗原
    Nlrp4e (NLRP4E) (NLR Family, Pyrin Domain Containing 4E (NLRP4E))
    别名
    Nlrp4e
    别名
    Nlrp4 Protein, Nalp4e Protein, Nalp-epsilon Protein, 4930406H16Rik Protein, NLR family, pyrin domain containing 4E Protein, Nlrp4e Protein
    背景
    NACHT, LRR and PYD domains-containing protein 4E (NALP-epsilon),FUNCTION: May be involved in inflammation and recognition of cytosolic pathogen-associated molecular patterns (PAMPs) not intercepted by membrane-bound receptors. {ECO:0000250}.
    分子量
    112.5 kDa
    UniProt
    Q66X19
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