Nitrate Reductase 抗体
NR
适用: Arabidopsis thaliana, Barley
WB
宿主: 兔
Polyclonal
unconjugated
3 images
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- 抗原
- Nitrate Reductase (NR) (Nitrate Reductase, Assimilatory (NR))
- 适用
- Arabidopsis thaliana, Barley
- 宿主
- 兔
- 克隆类型
- 多克隆
- 应用范围
- Western Blotting (WB)
- 产品特性
- Expected / apparent Molecular Weight of the Antigene: 103 kDa / 117 kDa
- 纯化方法
- serum
- 免疫原
- KLH-conjugated synthetic peptide derived from conserved domain in NADH-NR protein sequences including A.thaliana NR1 P11832, At1g77760 and NR2 P11035, At1g37130
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- 应用备注
- Recommended Dilution: 1 : 1000 (WB).
- 说明
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ECL based detection systems are adviced to use since to low signal intensity can be obtaied with BCIP/NBT system.For working with diatom samples ECL Advance (GE Healthcare) or other more sensitive ECL detection reagent is recommended.In Chlamydmonas reinhardtii anti-NR antibody is also reacting with L-Aminoacid Oxidase (a nitrogen scavenging enzyme induced during nitrogen starvation).
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- For reconstitution add 100 µL of sterile water
- 注意事项
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Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Once reconstituted make aliquots to avoid repreated freeze-thaw cycles. - 储存条件
- -20 °C
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- 抗原
- Nitrate Reductase (NR) (Nitrate Reductase, Assimilatory (NR))
- 背景
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AGI Code: At1g77760
Assimilatory nitrate reductase (NR, EC.1.6.6.1) catalyses the reduction of nitrate to nitrite in the cytoplasm. Plants contain 2 forms of NR: NADH-NR (most common form in plants and algae, predominantly found in green tissues) and NAD(P)H-NR (uses NADH or NADPH as the electron donor, constitutively expressed in plants at a low level). NADH-NR is a homodimer of two identical subunits (100-115 kDa each, hold together by a Mo-cofactor) each of them coded by up to three genes (NR1-3, NIA1-NIA3). - 分子量
- expected: 103 kDa , apparent: 117 kDa
- UniProt
- P11832, P11035
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