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Nitrate Reductase 抗体

NR 适用: Arabidopsis thaliana, Barley WB 宿主: 兔 Polyclonal unconjugated
产品编号 ABIN334562
发货至: 中国
  • 抗原
    Nitrate Reductase (NR) (Nitrate Reductase, Assimilatory (NR))
    适用
    Arabidopsis thaliana, Barley
    宿主
    克隆类型
    多克隆
    应用范围
    Western Blotting (WB)
    产品特性
    Expected / apparent Molecular Weight of the Antigene: 103 kDa / 117 kDa
    纯化方法
    serum
    免疫原
    KLH-conjugated synthetic peptide derived from conserved domain in NADH-NR protein sequences including A.thaliana NR1 P11832, At1g77760 and NR2 P11035, At1g37130
  • 应用备注
    Recommended Dilution: 1 : 1000 (WB).
    说明

    ECL based detection systems are adviced to use since to low signal intensity can be obtaied with BCIP/NBT system.For working with diatom samples ECL Advance (GE Healthcare) or other more sensitive ECL detection reagent is recommended.In Chlamydmonas reinhardtii anti-NR antibody is also reacting with L-Aminoacid Oxidase (a nitrogen scavenging enzyme induced during nitrogen starvation).

    限制
    仅限研究用
  • 状态
    Lyophilized
    溶解方式
    For reconstitution add 100 µL of sterile water
    注意事项
    Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
    Once reconstituted make aliquots to avoid repreated freeze-thaw cycles.
    储存条件
    -20 °C
  • 抗原
    Nitrate Reductase (NR) (Nitrate Reductase, Assimilatory (NR))
    背景
    AGI Code: At1g77760
    Assimilatory nitrate reductase (NR, EC.1.6.6.1) catalyses the reduction of nitrate to nitrite in the cytoplasm. Plants contain 2 forms of NR: NADH-NR (most common form in plants and algae, predominantly found in green tissues) and NAD(P)H-NR (uses NADH or NADPH as the electron donor, constitutively expressed in plants at a low level). NADH-NR is a homodimer of two identical subunits (100-115 kDa each, hold together by a Mo-cofactor) each of them coded by up to three genes (NR1-3, NIA1-NIA3).
    分子量
    expected: 103 kDa , apparent: 117 kDa
    UniProt
    P11832, P11035
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