Myc Tag 产品
(Myc Tag)
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Antibodies that are specific to the Myc tag are commonly used in molecular biology and cell biology research to detect and study proteins that have been tagged with the Myc epitope. The Myc tag is a short peptide sequence derived from the c-Myc protein, which is often genetically fused to a target protein of interest. This fusion allows researchers to track, purify, and study the behavior of the tagged protein within cells or in biochemical assays.
- Protein Expression and Localization: By fusing the Myc tag to a protein of interest, researchers can monitor the expression and subcellular localization of the tagged protein in cells. Antibodies against the Myc tag can then be used for immunofluorescence or immunohistochemistry to visualize where the tagged protein is located within the cell.
- Western Blotting: Antibodies against the Myc tag are used to detect the presence and relative expression levels of Myc-tagged proteins in cell lysates or tissue samples. This is achieved through Western blotting, a common technique in molecular biology that involves separating proteins by size and then using antibodies to detect specific proteins on a membrane.
- Co-Immunoprecipitation (Co-IP): Researchers often use Co-IP to study protein-protein interactions. By immunoprecipitating the Myc-tagged protein using an anti-Myc antibody, they can pull down its interacting partners and analyze them through subsequent assays.
- Affinity Purification: The Myc tag can be exploited to purify Myc-tagged proteins from cell extracts using affinity chromatography. An anti-Myc antibody is immobilized on a solid support, allowing selective binding and subsequent elution of the tagged protein for downstream analyses.
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Latest Publications for our Myc Tag Products
: "Exosomes and STUB1/CHIP cooperate to maintain intracellular proteostasis." in: PLoS ONE, Vol. 14, Issue 10, pp. e0223790, (2020) (PubMed).: "BRET-based RAS biosensors that show a novel small molecule is an inhibitor of RAS-effector protein-protein interactions." in: eLife, Vol. 7, (2019) (PubMed).
: "Critical relevance of genomic gains of PRL-3/EGFR/c-myc pathway genes in liver metastasis of colorectal cancer." in: Oncology letters, Vol. 17, Issue 1, pp. 1257-1266, (2019) (PubMed).
: "Striosome-based map of the mouse striatum that is conformable to both cortical afferent topography and uneven distributions of dopamine D1 and D2 receptor-expressing cells." in: Brain structure & function, Vol. 223, Issue 9, pp. 4275-4291, (2019) (PubMed).
: "A novel luciferase-based assay for the detection of Chimeric Antigen Receptors. ..." in: Scientific reports, Vol. 9, Issue 1, pp. 1957, (2019) (PubMed).
: "Abnormal Expression of c-Myc Oncogene in NK Cells in Patients with Cancer." in: International journal of molecular sciences, Vol. 20, Issue 3, (2019) (PubMed).
: "ER intrabody-mediated inhibition of interferon α secretion by mouse macrophages and dendritic cells." in: PLoS ONE, Vol. 14, Issue 4, pp. e0215062, (2019) (PubMed).
: "miR-708-5p promotes fibroblast-like synoviocytes' cell apoptosis and ameliorates rheumatoid arthritis by the inhibition of Wnt3a/β-catenin pathway." in: Drug design, development and therapy, Vol. 12, pp. 3439-3447, (2019) (PubMed).
: "Discovering metabolic disease gene interactions by correlated effects on cellular morphology." in: Molecular metabolism, Vol. 24, pp. 108-119, (2019) (PubMed).
: "Tumor angiogenesis is differentially regulated by phosphorylation of endothelial cell focal adhesion kinase tyrosines-397 and -861." in: Cancer research, (2019) (PubMed).
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