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Hep2 Whole Cell Lysate

Name: Hep2 Whole Cell Lysate
SKU: ABIN964023
Availability: OutOfStock

WB Whole Cell Lysate Cell Lysate HepG2 Cells 适用: 人

Coommassie stained SDS-PAGE of 20 µl of Human Derived Hep2 Whole Cell Lysate (Ready-to-Use) separated in a 4-20% gradient gel under reducing conditions (lane 2). (Hep2 Whole Cell Lysate)

SDS

1 image

(1 reference)

产品编号 ABIN964023

Supplier Product No.: w09-000-363

发货至: 中国

Quick Overview for Hep2 Whole Cell Lysate (ABIN964023)

宿主

人

应用范围

Western Blotting (WB)

Lysed Cells

HepG2 Cells

Lysate Type

Cell Lysate

Lysate Fraction

Whole Cell Lysate

资源

人细胞

Supplier Product No.

w09-000-363

Supplier

Rockland

特异性

Hep2 cells were grown in Dulbecco’s medium supplemented with 10% fetal bovine serum. Cells were washed with PBS and then incubated on ice in modified RIPA buffer, containing 150 mM sodium chloride, 50 mM Tris HCl, pH 7.4, 1 mM EDTA, 1.0% NP-40, 0.5% sodium deoxycholic acid , 0.1% SDS and 0.01% (w/v) sodium azide to lyse the cells. Protein integrity was ensured using a cocktail of protease inhibitors with broad specificity for the inhibition of aspartic, cysteine, and serine proteases as well as aminopeptidases (0.1 mM AEBSF HCl, 0.08 µM Aprotinin, 5 µM Bestatin, 1.5 µM E-64, 2 µM Leupeptin Hemisulfate, 1 µM Pepstatin A). Phosphatase inhibitors 1 mM NaF and 1 mM Na3VO4 were also added. Cell debris was removed by centrifugation. Protein concentration was determined by a modified Lowry assay using a commercially available kit. Protein concentration was adjusted to 2 mg/ml and then an equal volume of 2X SDS-PAGE sample buffer was added.

产品特性

Cell Line: Human HEp2 (HeLa contaminant)
Induction: None (Control)
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HSPD1 293T Cell Transient Overexpression Lysate(Denatured)
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HAL 293T Cell Transient Overexpression Lysate(Denatured)
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GYPA 293T Cell Transient Overexpression Lysate(Denatured)
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IMPDH1 293T Cell Transient Overexpression Lysate(Denatured)
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HLA-E 293T Cell Transient Overexpression Lysate(Denatured)
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MDM4 293T Cell Transient Overexpression Lysate(Denatured)
MDM4 WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells 适用: 人

MDM2 293T Cell Transient Overexpression Lysate(Denatured)
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MDK 293T Cell Transient Overexpression Lysate(Denatured)
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应用备注

Ready-to-use lysates are especially prepared as positive controls for separation by SDS-PAGE and subsequent western blot analysis. Lysates are prepared in denaturing buffer WITHOUT dissociating agents (i.e. no 2-mercaptoethanol or dithiothreitol has been added). Heat lysate to 95° C for 5 minutes and rapidly cool. If dissociating conditions are desired, add reducing agent prior to heating. The recommended loading volume per lane is 10-20 µl depending on the size format of your gel.

说明

Lysate Fractionation: Whole Cell Lysate
Lysate Stimulation: Not Stimulated
Lysate Tissue Culture: Tissue Culture

限制

仅限研究用
状态

Liquid

浓度

1.0 mg/mL

缓冲液

1X SDS-PAGE Sample Buffer (62.5 mM Tris HCl, 2% SDS, 10% Glycerol and 0.005% bromophenol blue, pH 6.8)

注意事项

Ready-to-use lysates are especially prepared as positive controls for separation by SDS-PAGE and subsequent western blot analysis. Lysates are prepared in denaturing buffer WITHOUT dissociating agents (i.e. no 2-mercaptoethanol or dithiothreitol has been added). Heat lysate to 95 °C for 5 minutes and rapidly cool. If dissociating conditions are desired, add reducing agent prior to heating. The recommended loading volume per lane is 10-20 µl depending on the size format of your gel.

储存条件

-80 °C

有效期

3 months
Laffy, Dodev, Macpherson, Townsend, Lu, Dunn-Walters, Fraternali: "Promiscuous antibodies characterised by their physico-chemical properties: From sequence to structure and back." in: Progress in biophysics and molecular biology, Vol. 128, pp. 47-56, (2017) (PubMed).
背景

Hep2 Whole Cell Lysate Ready-to-use whole cell lysates produced are derived from cell lines or tissues using highly refined extraction protocols to ensure exceptionally high quality, protein integrity and lot-to-lot reproducibility. All extracts are tested by SDS-PAGE using 4-20% gradient gels and immunoblot analysis using antibodies to key cell signaling components to confirm the presence of both high molecular weight and low molecular weight proteins.
Synonyms: Hep2, Lysate, Whole Cell Lysate, Hep2 Lysate