Adipose Subcutaneous Diabetic Disease Lysate
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- 资源
- Human
- 应用范围
- Western Blotting (WB)
- 产品特性
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Human subcutaneous adipose tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70 °C. The human subcutaneous adipose tissue total protein is provided in a buffer including HEPES ( pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the subcutaneous adipose tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The subcutaneous adipose tissue is then Western analyzed by either GAPDH or beta-actin antibody, and the expression level is consistent with each lot.
Source: Human Subcutaneous Adipose tissue
Diagnosis: Diseased
Tissue or Cell type: Adipose - Lysate Type
- Tissue Lysate
- Lysate Fraction
- Whole Tissue Lysate
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VNN1 293T Cell Transient Overexpression Lysate(Denatured)
VNN1 WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells 适用: 人
CYP26A1 293T Cell Transient Overexpression Lysate(Denatured)CYP26A1 WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells 适用: 人
APLP2 293T Cell Transient Overexpression Lysate(Denatured)APLP2 WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells 适用: 人
BIRC3 293T Cell Transient Overexpression Lysate(Denatured)BIRC3 WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells 适用: 人
BAG1 293T Cell Transient Overexpression Lysate(Denatured)BAG1 WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells 适用: 人
ATF7 293T Cell Transient Overexpression Lysate(Denatured)ATF7 WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells 适用: 人
PF4 293T Cell Transient Overexpression Lysate(Denatured)PF4 WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells 适用: 人
ZD52F10 Overexpression Lysate (Denatured)DMKN WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells 适用: 人
CLUHP3 Overexpression Lysate (Denatured)WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells 适用: 人
PGM3 293T Cell Transient Overexpression Lysate(Denatured)PGM3 WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells 适用: 人
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- 应用备注
- Adipose Subcutaneous lysate is for use in Western blotting, 10 μg to 20 μg per lane is recommended for mini gel.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 2 mg/mL
- 缓冲液
- HEPES pH 7.9, MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, and NP-40.
- 注意事项
- Repeated freezing and thawing is not recommended.
- 储存条件
- 4 °C/-80 °C
- 储存方法
- Store at 2-8 °C for continuous use. For extended storage, freeze working aliquots at -70 °C.
- 有效期
- 6 months
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