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DYKDDDDK Positive Control Lysate

PC, WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells 适用: 标记
产品编号 ABIN1607004
发货至: 中国
  • 抗原 See all DYKDDDDK Tag products
    DYKDDDDK Tag
    宿主
    标记
    资源
    人细胞
    应用范围
    Positive Control (PC), Western Blotting (WB)
    内毒素水平
    Low Endotoxin : No
    Lysed Cells
    HEK 293T Cells
    Lysate Type
    Overexpression Lysate
    Lysate Fraction
    Whole Cell Lysate
  • 应用备注
    Ready-to-use lysates are especially prepared as positive controls for separation by SDS-PAGE and subsequent western blot analysis. Lysates are prepared in denaturing buffer WITHOUT dissociating agents (i.e. no 2-mercaptoethanol or dithiothreitol has been added). If dissociating conditions are desired, add reducing agent prior to heating. Gently invert vial several times to ensure that the solution is homogeneous and that any precipitated material is re-dissolved. A loading volume of 0.25 to 2 µL is recommended for SDS-PAGE. The product is supplied in 1X loading buffer and is ready-to-use.
    限制
    仅限研究用
  • 状态
    Liquid
    缓冲液
    1X SDS-PAGE Sample Buffer (62.5 mM Tris HCl, 2 % SDS, 10 % Glycerol and 0.005 % bromophenol blue, pH 6.8)
    储存条件
    -80 °C
    储存方法
    Store vial at -70° C or COLDER. For extended storage, aliquot contents to minimize freeze/thaw cycles.
    有效期
    Expiration date is three (3) months from date of opening.
  • 抗原
    DYKDDDDK Tag
    Abstract
    DYKDDDDK Tag 产品
    物质类
    Tag
    背景
    This product is intended as a positive control for use with anti-DYKDDDDK tag antibodies.
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