(1 reference)-
- 抗原 See all dsDNA Ab products
- dsDNA Ab (Anti-Double Strand DNA Antibody (dsDNA Ab))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Competition ELISA
- 应用范围
- ELISA
- 原理
- dsDNA ELISA is intended for the detection of antibodies in human serum to dsDNA antigen and as an aid in diagnosis of Systemic lupus eruthematosus (SLE).
- 样品类型
- Serum
- Analytical Method
- Semi-Quantitative
- 特异性
- 97.9 %
- 灵敏度
- 100%
- 组件
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1. Microplate: wells coated with Purified dsDNA antigen
2. Serum Diluent Type II
3. Positive Control: Human serum or defibrinated plasma
4. Calibrator: Human serum or defibrinated plasma
5. Negative Control: Human serum or defibrinated plasma
6. HRP Conjugate: Goat anti-human IgG and IgM
7. Wash Buffer Type I 20X
8. Chromogen/Substrate Solution Type I
9. Stop Solution: 1N H2SO4 solution - 试剂未包括
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1. Precision pipettes
2. Distilled or deionized water
3. EIA kit Microplate Washer
4. EIA kit Microplate Reader with a (single wavelength 450 nm or dual 600-650)
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- 应用备注
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Notable features of Autoimmune Disease ELISA Kits:
- User-friendly directions and explanation of test procedures
- Simple and safe reagent preparation
- Clear instructions on specimen collection
- Comprehensive package of required materials
- Explicit quality control and storage guidelines
- Reliable and easy-to-read test results - 样本量
- 10 μL
- 实验时间
- 1.5 h
- 板类型
- Pre-coated
- 实验流程
- dsDNA elisa test is an Enzyme-Linked Immunosorbent Assay to detect antibodies to dsDNA antigen. Purified dsDNA antigen is attached to the wells. When antigens bound to the solid phase are brought into contact with a patient serum, antigen specific antibody, if present, will bind to the antigen in the wells forming antigen-antibody complexes. Excess antibody is removed by washing and followed by the addition of goat anti-human IgG, M conjugated with horseradish peroxidase which then binds to the antibody-antigen complexes.
- 限制
- 仅限研究用
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- 储存条件
- 4 °C
- 有效期
- 12-14 months
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: "Evaluation of anti-nuclear antibodies and kidney pathology in Lewis rats following exposure to Libby amphibole asbestos." in: Journal of immunotoxicology, (2012) (PubMed).
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: "Evaluation of anti-nuclear antibodies and kidney pathology in Lewis rats following exposure to Libby amphibole asbestos." in: Journal of immunotoxicology, (2012) (PubMed).
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- 抗原 See all dsDNA Ab products
- dsDNA Ab (Anti-Double Strand DNA Antibody (dsDNA Ab))
- 别名
- DsDNA Antibody (dsDNA Ab 产品)
- 物质类
- Antibody, Antibody
- 背景
- Autoimmune diseases are categorized into two general groups: One group is characterized by the production of tissue-specific autoantibodies, the other by autoimmune reactivity with normal cell nuclear and/or cytoplasmic antigens with no tissue type specificity. The latter group includes diseases such as mixed connective tissue disease, scleroderma, rheumatoid arthritis, dermatomyositis, polymyositis, SLE and Sjogren syndrome.
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