GFP Western Blot Kit: for GFP Chemiluminescent Western Blotting
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- 应用范围
- Dot Blot (DB), Western Blotting (WB)
- Supplier Product No.
- kca215
- Supplier
- Rockland
- 交叉反应
- recombinant GFP (rGFP), enhanced GFP (eGFP)
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- 应用备注
- GFP Western Blot Kit is ideal for detection of GFP-tagged recombinant proteins by western blot. The GFP Western Blot kit makes use of optimized anti-GFP antibody. The Chemiluminescent GFP Western Blot kit is useful for both western blotting and dot blotting methods. Please read the entire product insert prior to use.
- 说明
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Detection Kit Type: Chemiluminescent Western Blot Kit
- 限制
- 仅限研究用
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- 注意事项
- Wash buffers MUST NOT contain SODIUM AZIDE or other inhibitors of peroxidase activity!
- 储存条件
- RT/4 °C
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- 背景
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GFP Chemiluminescent Western Blot Kit allows for the detection of GFP-tagged recombinant proteins present in cell lysates provided by the user. After protein separation by SDS-PAGE and transfer, the membrane is probed with monoclonal Anti-GFP. Detection of the membrane bound antibody-antigen complex is achieved by the addition of a secondary antibody conjugated to the enzyme horseradish peroxidase. The enzyme reacts with a specialized formulation of luminol, an extremely sensitive, non-radioactive substrate that emits light and allows visualization using X-ray film or other imaging methods, including highly sensitive CCD cameras and imaging systems.
Synonyms: GFP Western Blotting Kit, GFP Chemiluminescent Kit, Green Fluorescent Protein Antibody Kit, Immunoblotting Kit, GFP, rGFP, eGFP
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