Anti-Nuclear Antibody (ANA) ELISA 试剂盒
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- 抗原 See all Anti-Nuclear Antibody (ANA) ELISA试剂盒
- Anti-Nuclear Antibody (ANA)
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Competition ELISA
- 应用范围
- ELISA
- Analytical Method
- Quantitative
- 产品特性
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ELISA kit for the detection of ANA in the research laboratory
Alternative Names: Anti-nuclear antibodies ELISA kit
Purified antigens (SSA/Ro, SSB/La, RNP 70, Sm, RNP/Sm, Scl70, centromere B and Jo1) are bound to microwells. Antibodies to these antigens, if present in diluted serum, bind in the microwells. Washing of the microwells removes unbound serum antibodies. Horseradish peroxidase (HRP) conjugated antihuman IgG immunologically binds to the bound patient antibodies forming a conjugate/antibody/antigen complex. Washing of the microwells removes unbound conjugate. An enzyme substrate in the presence of bound conjugate hydrolyzes to form a blue color. The addition of an acid stops the reaction forming a yellow endproduct. The intensity of this yellow color is measured photometrically at 450 nm. - Top Product
- Discover our top product ANA ELISA Kit
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Anti-Nuclear Antibody (ANA) ELISA Kit
ANA 适用: 小鼠 Colorimetric 7.8-500 pg/mL Plasma, Serum
Anti-Nuclear Antibody (ANA) ELISA KitANA 适用: 小鼠 Colorimetric Sandwich ELISA 0.46 ng/mL - 30 ng/mL Plasma, Serum, Tissue Homogenate
Anti-Nuclear Antibody (ANA) ELISA KitANA 适用: 人 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Plasma, Serum, Tissue Homogenate
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- 应用备注
- Optimal conditions to be determined by end-user
- 板类型
- Pre-coated
- 实验流程
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Purified antigens (SSA/Ro, SSB/La, RNP 70, Sm, RNP/Sm, Scl70, centromere B and Jo1) are bound to microwells. Antibodies to these antigens, if present in diluted serum, bind in the microwells. Washing of the microwells removes unbound serum antibodies. Horseradish peroxidase (HRP) conjugated antihuman IgG immunologically binds to the bound patient antibodies forming a conjugate/antibody/antigen complex. Washing of the microwells removes unbound conjugate. An enzyme substrate in the presence of bound conjugate hydrolyzes to form a blue color. The addition of an acid stops the reaction forming a yellow endproduct. The intensity of this yellow color is measured photometrically at 450nm.
- 限制
- 仅限研究用
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- 储存条件
- 4 °C
- 储存方法
- Store at 2-8 °C.
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- 抗原 See all Anti-Nuclear Antibody (ANA) ELISA试剂盒
- Anti-Nuclear Antibody (ANA)
- 别名
- anti-ANA (ANA 产品)
- 物质类
- Antibody, Antibody
- 背景
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Inflammatory connective tissue diseases are characterized by idiopathic genesis along with disturbances in terms of cellular and humoral immunity, systemic organ failure and a chronic course of disease. Additionally, connective tissue diseases exhibit overlapping symptomatic features that render an accurate diagnosis difficult. Considering the diversity of mixed connective tissue diseases, such disorders exhibit a common serological characteristic, the presence of antinuclear antibodies. These antibodies are directed against parts of the cell nucleus and the cytoplasm, and many rheumatic diseases are characterized by the presence of one or more of these ANAs. Antibodies to doublestranded DNA (dsDNA), singlestranded DNA (ssDNA), histone, nuclear ribonucleoprotein (RNP) and Smith antigen (Sm) are associated with SLE, while antibodies to Sjogren´s Syndrome A (SSA/Ro) and Sjogren´s Syndrome B (SSB/La) can occur in both SLE and Sjogren´s Syndrome (SS). Antibodies to Jo1 may be observed in polymyositis and dermatomyositis, while antibodies to sclerodermaassociated antigen (Scl70) and centromere can occur in patients with progressive systemic sclerosis (PSS). Antihistone antibodies are associated with SLE and druginduced lupus, while antiRNP antibodies are linked with mixed connective tissue disease (MCTD) and with SLE. Antibodies directed against centromere are associated with CREST syndrome.
Synonyms: Anti-nuclear antibodies ELISA kit.
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