Metanephrine ELISA 试剂盒
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- 抗原 See all Metanephrine (MN) products
- Metanephrine (MN)
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适用
- 激素
- 实验类型
- Competition ELISA
- 应用范围
- ELISA
- 产品特性
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ELISA kit for the detection of 2 MET in the research laboratory
Alternative Names: Metanephrin/Normetaphrin ELISA kit
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Metanephrine (MN) ELISA Kit
MN 适用: Various Species Colorimetric Competition ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Metanephrine (MN) ELISA KitMN 适用: 人 Colorimetric Competition ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Metanephrine (MN) ELISA KitMN 适用: 小鼠 Colorimetric Competition ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Metanephrine (MN) ELISA KitMN 适用: Various Species Colorimetric Competition ELISA 0.16 ng/mL - 10 ng/mL Cell Culture Supernatant, Plasma, Serum
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- 应用备注
- Optimal conditions to be determined by end-user
- 实验流程
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First, the plasma proteins are removed by precipitation. After this Metanephrine (Metadrenaline) and Normetanephrine (Normetadrenaline) are acylated. The subsequent competitive ELISA kit uses the microtiter plate format. The antigen is bound to the solid phase of the microtiter plate. The acylated standards, controls and samples and the solid phase bound analytes compete for a fixed number of antiserum binding sites. After the system is in equilibrium, free antigen and free antigenantiserum complexes are removed by washing. The antibody bound to the solid phase is detected by an antirabbit IgGperoxidase conjugate using TMB as a substrate. The reaction ismonitored at 450 nm. Determination of unknown samples is achieved by comparing their absorbance with a reference curve prepared with known standards. The antisera used in this test kit only recognise the biologically relevant Lforms of metanephrines. Commercially available synthetic normetanephrine or metanephrine is always a mixture of the Dand Lforms. The ratio between both forms differs widely from lot to lot. This has important implications if synthetic metanephrines are used to enrich native samples. As only about 50 % of the synthetic metanephrines the Lportion will be detected by use of this kit, spiked samples will be underestimated. Therefore native samples containing solely the Lform should be used.
- 限制
- 仅限研究用
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- 储存条件
- 4 °C
- 储存方法
- Store at 2-8 °C.
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- 抗原 See all Metanephrine (MN) products
- Metanephrine (MN)
- 别名
- Metanephrine (MN 产品)
- 物质类
- Chemical
- 背景
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Metanephrine is a metabolite of epinephrine created by action of catechol-O-methyl transferase on epinephrine. An article in the Journal of the American Medical Association, 2002, indicated that the measurement of plasma free metanephrines is the best tool in the diagnosis of pheochromocytoma, an adrenal medullary neoplasm. Normetanephrine is a metabolite of norepinephrine created by action of catechol-O-methyl transferase on norepinephrine. It is excreted in the urine and found in certain tissues. It is a marker for catecholamine-secreting tumors such as pheochromocytoma.
Synonyms: Metanephrin/Normetaphrin ELISA kit.
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